Objective Objective To explore the relations between serum epithelium-derived factor and insulin resistance in type2 diabetes mellitus. Methods Using enzeyme-linked immunosorbent assay (ELISA) to detect serum PEDF in 52 normal glucose tolerance people(NGT) and 124 type 2 diabetes mellitus (17DM) patients. FCP, 2hCP,HbAlc,blood glucose,blood lipid and highly sensitive C-reactive protein (hs-protein) were simultaneously determined. Results (1) The serum PEDF( sPEDF) levels of T2DM were significantly higher than of NGT group [2893.45(1641.91-5533.13) μg/L vs 2672.95 (1486.544039.13) μg/L (P <0.05)]. (2) Quartiles of the concentration of sPEDF were computed. Compared with those in the lowest quartile of sPEDF, type 2 diabetes mellitus patients in the highest quartile had higherHOMA-IR values [1.90(1.60-2.70) vs 1.45(0.90-2.10) (P <0.01) ]. (3) Bivariate correlation showed that sPEDF was positively correlated with HOMA -IR,SBP,DBP,FCP,hs-CRP (r = 0.206,0.210,0.170,0.213,0.221,P<0.05 or 0.01). (4) Multiple linear regression analysis revealed that HOMA -IR (p=0.174,P<0.05),SBP (p=0.189,P<0.05) were the independent factors of sPEDF. Conclusion The serum PEDF level o.f Chinese population with type 2 diabetes increased significantly, and the serum PEDF level is independently related to HOMA-IR.%目的 探讨血清色素上皮源性因子(sPEDF)与2型糖尿病(T2DM)胰岛素抵抗的关系.方法 采用酶联免疫法检测176例受试者[糖调节正常组(NGT) 52例,T2DM 124例]的sPEDF水平,同时检测空腹C肽(FCP)、2小时C肽(2h CP)糖化血红蛋白(HbA1c)、血糖、血脂、高敏C反应蛋白(hs-CRP)等生化指标的水平.结果 (1)T2DM组sPEDF水平显著高于NGT组[2893.45( 1641.91～ 5533.13) μg/L vs 2672.95( 1486.54～4039.13) μg/L(P＜0.05)]；(2)按sPEDF水平四分位分组,上四分位点的胰岛素抵抗指数(HO-MA-IR)值显著高于下四分位点者[1.90(1.60 ～2.70)vs 1.45(0.90～2.10)(P＜0.01)]；(3)单变量相关关系分析显示:sPEDF水平与HOMA -IR、SBP、DBP、FCP、hs-CRP呈显著正相关(r=0.206,0.210,0.170,0.213,0.221,P＜0.05或0.01)；(4)逐步多元回归显示HOMA - IR(β =0.174,P＜0.05),SBP(β=0.189,P＜0.05)是sPEDF的独立影响因素.结论 中国人群中2型糖尿病患者sPEDF水平显著升高,与胰岛素抵抗指数显著独立相关.
展开▼
