Objective To study the expression levels of p27 and proliferating cell nuclear antigen (PCNA) in thyroid carcinoma tissues and their correlation. Methods The immunohistochemistry staining was used to assay 86 cases of thyroid carcinoma,54 cases thyroid adenoma and 54 cases normal tissue adjacent thyroid carcinoma. Results The positive rate of p27 in the thyroid carcinoma was 37. 21% , which was higher than those in thyroid adenoma and normal tissue with significant difference ( x2 =22. 20, P < 0. 01). The positive rate of PCNA in the thyroid carcinoma was 66. 27% ,which was higher than those in the thyroid adenoma and normal tissue with significant difference (P < 0. 01). The positive rate of p27 in the Papillary carcinoma, follicular carcinoma, medullary carcinoma and undifferenti-ated carcinoma were 48. 94% ,31. 25% ,14. 29% and 11. 11% with significant difference(x2 =8. 85 ,P <0. 05) ;the positive rates of PCNA in the Papillary carcinoma, follicular carcinoma, medullary carcinoma and undifferentiated carcinoma were 53. 19% ,75% ,85. 71% and 88.89% with significant difference(x2 = 8. 57,P <0. 05). There was a negative correlation between the expressions levels of p27 and PCNA in the thyroid carcinoma (r= - 0. 51, P < 0. 01 ) . Conclusion The expression levels of p27 were reduced significantly in the thyroid carcinoma, and varied with the reduction of differentiation degree; the expression levels of PCNA were increased in thyroid carcinoma, and the positive expression levels were decreased with increasing degree of differentiation. Additionally, the expression levels of p27 and PCNA was negative correlation in the thyroid carcinoma.%目的 探讨甲状腺癌组织中p27和增殖细胞核抗原(PCNA)的表达情况及其相关性.方法 用免疫组织化学法检测86例甲状腺癌(甲状腺癌组)、54例甲状腺腺瘤(腺瘤组)和瘤旁正常甲状腺组织(正常组)中p27和PCNA的表达情况.结果 p27在甲状腺癌组阳性表达率分别为37.21％,高于腺瘤组和正常组,差异有统计学意义(P＜0.01).PCNA在甲状腺癌组阳性表达率为66.27％,高于腺瘤组和正常组,差异有统计学意义(x2 =22.16,P＜0.01).p27在甲状腺乳头状癌、滤泡癌、髓样癌和未分化癌中的阳性表达率分别为48.94％、31.25％、14.29％和11.11％,差异有统计学意义(x2=8.85,P＜0.05);PCNA在乳头状癌、滤泡癌、髓样癌和未分化癌中的阳性表达率分别为53.19％、75％、85.71％和88.89％,差异有统计学意义(x2=8.57,P＜0.05).甲状腺癌p27和PCNA表达呈负相关(r=-0.51,P＜0.01).结论 p27和PCNA在甲状腺癌中的阳性表达明显减少,并且随着分化程度降低减少更为明显;而PCNA在甲状腺癌中的阳性表达明显增加,并且随分化程度降低增加更为明显;并且p27和PCNA表达呈负相关.
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