Objective:To explore the detect in efficiency of Krebs von den Lungen-6 (KL-6) by latex agglutination method and evaluate its diagnostic value in patients with interstitial lung disease.Methods:The serum KL-6 levels of 44 healthy subjects were detected,and the reference interval provided by the kit manufacturer was verified.Serum KL-6 levels were compared between the ILD group,the other lung diseases group and the healthy subjects group.The diagnostic value of KL-6 for interstitial lung disease (ILD) was evaluated by drawing the ROC curve.Results:The serum KL-6 level of 43 in 44 healthy people (＞90％) was within the reference range provided by the manufacturer.The median concentrations of KL-6 in the ILD group,the other lung diseases group and the healthy subjects group were 824 (187.9-8 214),246 (101-2 129),and 168 (98-416) U/mL,respectively,and the differences were statistically significant (P＜0.001).The sensitivity and specificity of KL-6 in diagnosis of ILD was 75％ and 89.8％,respectively,with 500 U/mL as the cutoff value.The area under the ROC curve (AUC) of KL-6 for ILD diagnosis was 0.852 (95％ CI 0.793-0.911,P＜0.0001).Conclusions:The latex agglutination method for detecting serum KL-6 has good performance and KL-6 is a potential diagnostic index of ILD.%目的:探讨乳胶凝集法检测唾液酸化糖链抗原KL-6的性能,并评估KL-6对间质性肺病的诊断价值.方法:检测44例表面健康人血清KL-6水平,验证试剂盒厂商提供的参考区间.比较间质性肺病(interstitial lung disease,ILD)组、其他肺病组和表面健康人组血清KL-6水平,通过绘制ROC曲线评价KL-6对ILD的诊断性能.结果:44例表面健康人血清KL-6水平有43例在厂商提供的参考范围内(＞90％),通过验证.ILD组、其他肺病组和表面健康人组KL-6中位浓度分别为824(187.9～8 214)、246(101～2 129)、168(98～416) U/mL,差异有统计学意义(P＜0.001).以500U/mL为临界值,KL-6诊断ILD灵敏度和特异度分别为75％、89.8％.KL-6诊断ILD的ROC曲线下面积(AUC)为0.852(95％CI 0.793～0.911,P＜0.000 1).结论:乳胶凝集法检测血清KL-6的性能符合要求;KL-6对ILD具有较好的诊断效能,是潜在的诊断指标.
展开▼
