目的：：观察体外培养的髓核细胞在低氧及不同流体静压下凋亡的变化。方法：体外培养人退变髓核细胞,根据低氧环境随机分成2组：低氧组和正常氧分压组；然后根据不同流体静压条件再随机分为3组：不加压对照组、加压50 kPa 组和加压100 kPa 组；用 MTT 和末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记(terminal dexynucleotidyl transferase-mediated dUTP nick end labeling,TUNEL)测定法检测髓核细胞凋亡的变化。结果：低氧状态下髓核细胞的存活率较正常氧分压状态高,凋亡细胞减少。在不同流体压力下,50 kPa 压力组髓核细胞的凋亡阳性率与对照组相比,差异无统计学意义(P >0.05)；100 kPa 压力组比对照组髓核细胞凋亡增加31.65%,差异有统计学意义(P <0.01)。结论：低氧和低压力环境可以减少髓核细胞的凋亡。%Objective:To observe the change of apoptosis of nucleus pulposus cells cultured in vitro under hypoxic condition and different hydrostatic pressure.Methods:The human degenerative nucleus pulposus cells were cultured in vitro and randomly divided into 2 groups according to hypoxic condition:the hypoxia group and the normal oxygen partial pressure group.While the nucleus pulposus cells were randomly divided into three groups according to different hydrostatic pressure:the control group without pressure,the 50 kPa pressure group and the 100 kPa pressure group.The change of apoptosis of nucleus pulpo-sus cells was detected by methyl thiazolyl tetrazolium(MTT)method and terminal dexynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)assay.Results:As compared with that in normal oxygen partial pressure group,the proportion of survival nucleus pulposus cells in hypoxic group increased,while the number of apoptotic cells decreased.Under different hy-drostatic pressure,there was no statistically significant difference regarding the apoptotic positive rate of nucleus pulposus cells between the 50 kPa pressure group and the control group (P >0.05 ).However,the apoptosis positive rate in the 100 kPa pressure group increased by 3 1 .65%,compared with that in the control group,and the difference was statistically significant (P <0.01).Conclusions:Hypoxic condition,as well as low pressure condition,can reduce the occurrence of apoptosis of nu-cleus pulposus cells.
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