目的:探究肝癌细胞中的分泌性富含半胱氨酸的酸性蛋白(secreted protein acidic and rich in cysteine,SPARC)的表达与糖酵解作用的关系。方法:用 SPARC 质粒和 SPARC siRNA 分别转染人肝癌细胞株 HepG2,转染后采用比色法检测细胞的葡萄糖摄取量以及乳酸生成量的变化。结果:转染 SPARC 质粒后的 HepG2细胞的葡萄糖摄取量和乳酸生成量较对照组下降(P <0.05);转染 SPARC siRNA 后的 HepG2细胞的葡萄糖摄取量和乳酸生成量较对照组增加(P <0.05)。结论:在肝癌细胞中,SPARC 对糖酵解过程具有负性调控作用。%Objective:To investigate the relationship between the expression of secreted protein acidic and rich in cysteine (SPARC)and the glycolysis in hepatocarcinoma cells.Methods:HepG2 hepatocarcinoma cells were transfected with SPARC plasmid and SPARC siRNA,respectively.The change of glucose uptake and lactate generation after transfection was assayed by colourimetry method.Results:The glucose uptake and lactate generation decreased in HepG2 transfected with SPARC plas-mid,compared with the control group (P <0.05).However,the glucose uptake and lactate generation increased in HepG2 transfected with siRNA SPARC,compared with the control group(P <0.05).Conclusions:SPARC negatively regulates gly-colysis in hepatocarcinoma cells.
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