Objective To study the effects of simple portal hypertension on the endotoxin levels in serum and intestinal mucosa of rats. Methods A total of 16 rats were divided into the blank control group (4 rats) and the model groups (3-day group, 7-day group and 10-day group, 4 rats in each group). The rat model of partial portal vein ligation was established in the model groups, and samples of blood and jejunum, ileum and colon of the rats were taken on the 3rd, 7th and 10th days, respectively. Changes in the serum endotoxin levels were detected by ELISA. Histopathological changes of the intestinal tissues were examined by HE staining. Results The rat model of partial portal vein ligation was successfully established in all the model groups. The serum levels of endotoxin on the 3rd, 7th and 10th days in the model groups were not significantly different from that in the normal control group. Damages of different intestinal segments were not serious on the 3rd day after modeling. However, on the 7th day after modeling, there were some sowllen and damaged intestinal villi in the intestinal mucosa of each intestinal segment, and the connection between the epithelial cells and the lamina propria was broken, compared with that at 3 days after modeling. In addition, there were obvious damages in the intestinal mucosa and lamina propria on the 10th day, compared with that at 3 d and 7 d after modeling. Conclusions In the case of normal liver function, portal hypertension can cause intestinal mucosal damages within a short period of time, but the amount of endotoxin produced by intestine does not exceed the processing capacity of the liver and thus does not cause an increase in the serum endotoxin level.%目的 研究大鼠单纯门脉高压对血清内毒素以及肠黏膜的影响.方法 选取16只大鼠,分为空白对照组(4只)和模型组(3d组、7d组、10d组,每组4只),模型组构建门脉部分结扎模型,分别在3d、7d、10d采集血液及大鼠的空肠、回肠和结肠,采用ELISA法检测血清内毒素含量观察内毒素的变化情况,采集各组不同部位肠组织进行HE染色,进行病理组织学观察分析.结果 模型组大鼠 均成功构建了门静脉部分结扎模型.三个不同时间点血清内毒素的结果与正常对照组之间差异无显著性.成模3d后各肠段还未发生很大的损伤; 成模7d后各肠段较3d粘膜层的局部肠绒毛出现了部分破坏和 肿胀,上皮细胞与固有层间连结出现了松散的状况; 成模10d较3d和7d的肠粘膜层、固有层均出现了明显的损伤.结论 在肝功能正常的情况下,门脉高压短时间内可以造成肠黏膜的损伤,但肠道产生的内 毒素不会超出肝脏的处理能力而出现血清内毒素的升高现象.
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