Objective To compare the effect of silica-extraction method and Silico membrane based method in DNA purification from bones and teeth.Methods DNA samples were purified respectively with the silica-extraction method and MinElute PCR Purification kit from 6 bones and 8 teeth,then tested STR types by GlobalFiler? kits. And evaluated the two methods with the success rate and the peak height. Results Both of the two purification methods can successfully obtain the STR markers of the 14 samples. And there was no statistical difference between the two methods in the average peak height from bones and teeth. Conclusion The Silico membrane based method which have more advantages in operation is an efficient method to purify DNA from bones and teeth, and there is no significant difference compared with the silica-extraction method. But the cost is higher. It can be selectively used in forensic practice.%目的 比较硅珠法和硅胶膜法对骨骼和牙齿的纯化效果.方法 选择6根骨骼和8颗牙齿,进行消化后分别采用硅珠法与硅胶膜法进行纯化,用GlobalFiler?试剂盒进行扩增检测,通过比较检出率和峰高来评价这两种方法.结果 两种纯化方法均成功检测出了骨骼和牙齿的STR分型.同一样本中,相同基因座上两种方法检出的等位基因分型结果完全一致.两种方法处理得到的平均峰高差异无统计学意义.结论 硅胶膜法在骨骼及牙齿的纯化中能满足实际常规检案的要求,且和硅珠法无明显差异,但在操作上更具优势,缺点是成本较高,在实际工作中可以选择使用.
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