目的 通过扩增蝇类CO Ⅰ基因片段,结合形态学特征鉴定嗜尸性蝇类的种类.方法 运用改良平衡酚—tris饱和酚法提取蝇类DNA,进行CO Ⅰ序列扩增和测序,与数据库序列进行比对和分析.结果 改良平衡酚-tris饱和酚提取DNA方法可获得有效的蝇类DNA,并可应用于CO Ⅰ基因片段扩增,进而进行蝇类种类的鉴定.结论 平衡酚-Tris饱和酚法提取的噬尸性蝇类虫体DNA作为模板,用于CO Ⅰ序列扩增,测序后与数据库目的序列分析比对,可准确鉴定嗜尸性蝇类的种类;和传统的昆虫形态学特征鉴定方法比较,该体系更准确,应用范围更广.%Objective To identify the species of sarcosaphagous flies by amplifying cytochrome oxidase subunitI (CO I) gene fragment,combined with morphological characteristics.Methods The DNA of sarcosaphagous flies was extacted by modified Tris balanced phenol-Tris saturated phenol protocol,the amplificationand sequencing of CO Ⅰ fragment were conducted,and the results were then compared with the database for analysis.Results The modified DNA extration method by Tris balanced phenol-Tris saturated phenol could obtain effective DNA of sarcosaphagous flies,and be applied for CO Ⅰ fragment amplification,and thus for identification of the species of sarcosaphagous flies.Conclusion The DNA extracted from sarcosaphagous flies by modified Tris balanced phenol-Tris saturated phenol protocol could be used astemplate for the amplification of CO Ⅰ fragment,and the system could identify the species of sareosaphagous flies after sequencing and alignment with the sequences of database.Compared with traditional identification methods of using morphological characteristics,the current system is more accurate and could be more widely applied.
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