毛细管电泳是一种在生命科学应用最为广泛的生物分子分离技术,目前已广泛应用于DNA测序,SNP检测,DNA片段分析等.筛分介质是毛细管电泳性能的关键因素,筛分介质的种类、结构、分子量及所形成的溶液决定着生物分子的迁移特性、分辨率、读出长度、重现性等参数,对分离结果有重大影响.本文对近年来用于DNA分离的无胶筛分介质的研究和应用情况进行综述,希望能对相关研究和鉴定提供参考和借鉴.%Capillary electrophoresis technology, as one kind of biomolecule separation methods in life science, has been widely used in DNA sequencing, SNP and DNA fragment analysis, etc. Sieving media is a key factor in the performance capillary electrophoresis, and its category, conformation and related property will determine the migration characteristics of biological molecules, resolution, length of read and reproducibility. Therefore, sieving media has a significant influence on the separation results. A review on polymers used as DNA separation media in capillary electrophoresis is presented.
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