背景：糖尿病（ DM）胃肠动力障碍的机制目前尚不明确，越来越多的研究认为胃肠平滑肌肌源性因素在该病的发生中起重要作用。近年RhoA/ROCK信号通路在DM并发症中的作用成为研究热点。目的：分析DM患者结肠肌层中RhoA/ROCK信号通路主要信号分子的表达变化，探讨该信号通路在DM结肠动力障碍中的可能作用。方法：收集2012年9月-2013年12月南京医科大学第一附属医院行结肠癌根治术患者的正常结肠组织，根据糖化血红蛋白水平，将患者分为DM组和对照组。采用免疫组化或蛋白质印迹法检测结肠肌层中RhoA/ROCK信号通路主要信号分子RhoA、ROCK1、MYPT1和p-MYPT1的表达情况。结果：免疫组化结果显示DM组结肠肌层中RhoA表达明显低于对照组，差异有统计学意义（ P＜0.05）；蛋白质印迹法结果显示，与对照组相比，DM组RhoA、ROCK1和p-MYPT1蛋白表达均明显减少（0.62±0.42对1.15±0.69，0.54±0.09对0.75±0.05，0.70±0.28对1.04±0.47；P＜0.05），而MYPT1蛋白表达差异无统计学意义（0.94±0.50对1.21±0.80，P＞0.05）。结论：DM患者结肠肌层中RhoA/ROCK信号通路活性被抑制，可能与DM结肠动力障碍有一定相关性。%BacKground:The mechanism of diabetic gastrointestinaI dysmotiIity is stiII uncIear. More and more studies showed that gastrointestinaI smooth muscIe derived factors pIay an important roIe in gastrointestinaI dysmotiIity. RecentIy,the roIe of RhoA/ROCK signaIing pathway in diabetic compIications become a research hotspot. Aims:To investigate the possibIe roIe of RhoA/ROCK signaIing pathway in diabetic coIon dysmotiIity by examining the expressions of major signaIing moIecuIes in diabetic coIon muscIe. Methods:NormaI coIon tissue sampIes taken from patients undergoing radicaI surgery for coIonic cancer from Sept. 2012 to Dec. 2013 at the First AffiIiated HospitaI of Nanjing MedicaI University were coIIected. According to gIycated hemogIobin IeveI,the patients were divided into diabetes meIIitus( DM)group and controI group. The expressions of major signaIing moIecuIes in RhoA/ROCK1 signaIing pathway incIuding RhoA,ROCK1,MYPT1 and p-MYPT1 were determined by immunohistochemistry or Western bIotting. Results:Immunohistochemistry showed that expression of RhoA protein in DM group was significantIy Iower than that in controI group(P<0. 05). Western bIotting showed that expression IeveIs of RhoA,ROCK1 and p-MYPT1 proteins were significantIy Iower in DM group than in controI group(0. 62 ± 0. 42 vs. 1. 15 ± 0. 69,0. 54 ± 0. 09 vs. 0. 75 ± 0. 05,0. 70 ± 0. 28 vs. 1. 04 ± 0. 47;P<0. 05),whereas no significant difference in expression IeveI of MYPT1 protein was found between DM group and controI group(0. 94 ± 0. 50 vs. 1. 21 ± 0. 80,P>0. 05). Conclusions:The inhibition of RhoA/ROCK signaIing pathway in diabetic coIon muscIe may have some correIation with diabetic coIon dysmotiIity.
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