Objective To study the protective effect and find out the molecular mechanism of lycopene on damaged endothelial cells exposed to PM2.5.Methods The optimum concentration of lycopene was found , the endothelial cells exposed to 80 μg/ml PM2.5 were administrated by series of concentrations (0, 40, 80, 160μg/ml), cell viability was assayed by CCK-8 kits;MDA content and SOD activity were elevated respectively .HIF-1αexpression was detected by Western blot;IL-6 concentration was assayed by q-PCR and ELISA .Results The concentration of 80μg/ml lycopene rescued oxidative damage on endothelial cells exposed to 80μg/ml PM2.5.Moreover, SOD activ-ity was significantly increased 48.04%(P<0.05) and MDA concentration was significantly declined 38.89% (P<0.05) post-administra-tion;Furthermore, IL-6 and HIF-1αlevels in endothelial cells exposured to PM 2.5 were declined by lycopene .Conclusions Lycopene could rescue endothelial cells oxidative damage by exposure to PM 2.5.%目的：探讨番茄红素对PM2．5暴露内皮细胞氧化损伤的分子机制。方法利用CCK－8明确番茄红素减轻 PM2．5暴露内皮细胞损伤的最适宜浓度；检测细胞丙二醛（ MDA）含量及超氧化物歧化酶（ SOD）活性，评估番茄红素解除PM2．5对内皮细胞氧化损伤的作用；Western 印迹检测细胞内缺氧诱导因子（HIF）－1α表达；q－PCR、ELISA检测细胞白介素（IL）－6表达水平。结果解除因PM2．5暴露造成氧化损伤的最佳番茄红素浓度为80μg／ml；番茄红素作用使PM2．5暴露内皮细胞氧化应激指标SOD活性升高48．04％，MDA含量下降38．89％，IL－6、HIF－1α恢复到正常细胞水平。结论番茄红素具有解除PM2．5暴露内皮细胞氧化损伤的作用。
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