目的 研究乙酰肝素酶(HPSE)和血管内皮生长因子(VEGF)-C mRNA的表达是否与肺癌的侵袭性生长和淋巴结转移有关.方法 应用RT-PCR技术检测HPSE和VEGF-C mRNA在65例肺癌组织中的表达,并与癌旁组织和正常肺组织对比,同时结合肺癌的临床病理学特征及预后进行分析.结果 肺癌组织中HPSE和VEGF-C mRNA的阳性表达率明显高于正常肺组织和癌旁组织(P＜0.05)；不同病理类型肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P＞0.05)；不同分化程度肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P＞0.05)；Ⅲ期和Ⅳ期肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于Ⅰ期和Ⅱ期(P＜0.05)；生存3年以下的肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于生存3年以上者(P＜0.05).结论 肺癌的发生、发展可能与HPSE促血管生成和VEGF-C促淋巴管生成的协同作用有关；HPSE和VEGF-C基因表达可作为判断肺癌生物学行为和患者预后的一个参考指标.%Objective To study the expressions of HPSE and mRNA with invasiveness and metastasis in human lung cancer. Methods Expressions of HPSE and VEGF-C mRNA in 65 cases of lung cancer, adjacent tissues of cancer and normal tissues were tested by RT-PCR and analyzed by clinicopathological characteristics and prognosis of lung cancer. Results The positive rate of HPSE and VEGF-C mRNA in tumor tissues were significantly higher than those in adjacent tissues of cancer and normal tissues (P<0.05). There was not significant difference between HPSE and VEGF-C mRNA expressions and the type and grade of the tumor (P > 0.05). But the expressions of HPSE and VEGF-C mRNA were correlated with the clinical stage and survival time of the patients (P<0. 05). Conclusions Overexpres-sions of HPSE and VEGF-C mRNA in lung cancer tissues play role in occurrence and development of lung cancer. The expression of HPSE and VEGF-C mRNA can be used as an useful marker of the biological behavior of lung cancer and an independent prognosis factor for the patients with lung cancer.
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