Objective To observe the phosphorylation of tau protein after the step up of cholesterol in hippocampus and investigate the possible function of cholesterol in Alzheimer's disease (AD). Methods Rats were sieved with morris water maze for 7 days, injected with different dosage of cholesterol into hippocampus. Then ethology, abnormal tau phosphorylation and cholesterol level were examined by morris water maze, Western blot and immunohistochemistry. Results After injected 72 h with different dosage of cholesterol into hippocampus, the water maze latency of middle and high dose group were obvious extended (P <0. 01), the phosphorylation level of PHF-1 were increased ( P < 0.01), tau-1 were decreased (P < 0. 01), total tau (R1 34d) were no change. Conclusions The raise of cholesterol in brain which inducing the step up of tau phosphorylation could be a possible way to form AD.%目的 观察海马胆固醇含量升高后tau蛋白磷酸化程度的变化,探讨其在阿尔茨海默病(AD)中可能的作用.方法 水迷宫训练大鼠7d筛选合格大鼠后,海马直接注射不同剂量胆固醇,72 h后水迷宫检测行为学变化,并以免疫印迹和免疫组织化学方法检测大鼠海马tau蛋白磷酸化水平.结果 注射胆固醇后72 h,中高剂量组大鼠水迷宫潜伏期明显延长(P＜0.01),tau蛋白PHF-1位点磷酸化水平明显增高(P＜0.01),tau-1明显降低(P＜0.01),总tau蛋白(R134d)无明显变化.结论 海马胆固醇水平升高可导致tau蛋白磷酸化程度增加,可能参与AD的形成.
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