目的 探讨建立大鼠脂肪肝动物模型的方法.方法 60只雄性SD大鼠随机分成3组:正常对照(NS)组每日灌胃生理盐水和普通饲料喂养,高剂量模型(HM)组和低剂量模型(LM)组每天按照5 g/kg体重对大鼠灌胃浓度为 60%(V/V)和30%(V/V)的乙醇和按照5 ml/kg灌胃脂肪乳剂的方法,连续3 w,分别在第2周末和第3周末随机选取各组大鼠10只,观察其肝脏病理改变及血液生化指标的变化.结果 造模2 w可见轻度的肝脂肪变.造模3 w后,模型组大鼠肝脏重量明显升高,与正常组比较有显著性差异;病理表现为广泛的脂肪变性,血清三酰甘油(TG)、丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转氨酶(AST)、总胆固醇(TC)升高,高密度脂蛋白胆固醇(HDL-C)显著降低,与正常组比较差异有显著意义(P<0.05);血糖和透明质酸(HA)变化不大(P>0.05).结论 本造模方法与人类脂肪肝病变类似,方法简单易行,实验周期短,模型稳定可靠.%Objective To establish alcoholic fatty liver model with alcohol and fat emulsion in rats. Methods Sixty SD male rats were randomly divided into NS (intragastric infusion of saline, 5 ml/kg, fed with common diet), HM and LM groups (intragastric infusion of alcohol by 60％ V/V or 30％ V/V ,5 ml/kg , fed with intralipid, 5 ml/kg). Half of rats were sacrificed after 2 w to evaluate the process of alcoholic fatty liver formation. All rats were sacrificed at the end of the third week. The pathologic of the liver and the biochemistry index of plasma were observed. Results Slight fatty deposition was observed at the end of the 2nd week after experiment, and 3 weeks later, severe fatty deposition appeared. The levels of serum TG, CH, ALT and AST of HM and LM groups were higher than those of NS group(P＜ 0. 05 ). Conclusions The lesion of the alcoholic fatty liver model in rats is similar to that in humans. The experimant is very simple and the experiment cycle is short and the conclusion is clear.
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