Objective To investigate the correlations between toe brachial index (TBI) and Thl7 cells and Thl7-associated cyto-kines in old metabolic syndrome patients. Methods According to the standard of metabolic syndrome presented by Chinese Diabetes Society in. 2004, 161 metabolic syndrome patients were eligible recruited. TBI was observed using VP-1000 automatic atherosclerosis analyzer, and then the patients were divided into normal TBI group and lower TBI group, respectively. Frequencies of peripheral blood Thl7 cells were detected using flow cytometry. And ELISA was used to test serum IL-17, IL-6 and TNF-a. Results The frequency of peripheral blood Thl7 cells in lower TBI group was higher than that in normal TBI group (J = 7. 116, P < 0. 01). Levels of serum IL-17, IL-6 and TNF-a in lower TBI group were elevated than those in normal TBI group ( P < 0. 01). Thl7 cells frequency, IL-17, IL-6 and TNF-a were negatively correlated with TBI ( r = - 0. 443, - 0.485, - 0. 348 and - 0. 429, P < 0. 001). Multiple linear regression analysis showed that Th 17 cells frequency, IL-17, IL-6 and TNF-a were risk factors of TBI. Conclusions TBI is associated with Thl7 cells and Thl7-associated cytokines in old metabolic syndrome patients.%目的 探讨老年代谢综合征患者趾臂指数(TBI)与Th17细胞及其与细胞因子白细胞介素-17(IL-17)、白细胞介素-6(IL-6)及肿瘤坏死因子α(TNF-α)的相关性.方法 依据2004年中国糖尿病学会(CDS)提出的标准入选161例代谢综合征患者,应用全自动动脉硬化测定仪VP1000测定患者的TGBI,将患者分为TBI正常组和TBI降低组.采用流式细胞检测技术检测患者外周血中Th17细胞水平,并采用ELISA法检测所有高血压患者的血清IL-17、IL-6及TNF-α浓度水平.结果 TBI降低组外周血Th17细胞比例明显高于TBI正常组(t=7.116,P＜0.01).TBI降低组Th17细胞因子IL-17、IL-6及TNF-α血清浓度较TBI正常组显著升高(P＜0.01).相关及回归分析显示,TBI与外周血Th17细胞比例、血清IL-17、IL-6及TNF-α血清浓度水平呈负相关(r=-0.443、-0.485、-0.348及-0.429,均P＜0.001),Th17细胞比例、IL-17、IL-6、TNF-α血清浓度水平是影响baPWV的主要因素.结论 老年代谢综合征患者TBI与Th17细胞及其细胞因子相关.
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