Objective: To explore effect of atorvastation on expression of thymic stromal lymphopoietin ( TSLP ) in primary HU-VEC induced by ox-LDL. Methods:HUVECs mixed with ox-LDL ( 50 mg/L ) for 12 hours were divided into three groups: the control group was treated with PBS; Al group was added atorvastatin( 1 |xmol/L ); A2 group was added atorvastatin( 10 |xmol/L ). At 6 h and 12 h, TSLP protein in supernatant and cells was assessed by ELISA and immunofluorescence( IF ), TSLP mRNA in HUVECs was detected by RT-PCR. Results:The control group expressed high TSLP. Incubation of HUVEC with atorvastatin resulted in a significant decrease of TSLP mRNA and protein release from HUVEC, which was in a dose-dependent manner. The same results were showed a-bout TSLP protein in supernatant and cells. Conclusion: Atorvastatin can inhibited the expression of TSLP in cultured vascular endo-thelial cells induced by ox-LDL, which may be one of the mechanisms that statins inhibit inflammation in atherosclerosis.%目的:观察阿托伐他汀对ox-LDL诱导人脐静脉内皮细胞(HUVECs)表达胸腺基质淋巴细胞生成素(TSLP)的影响.方法:将HUVECs分为3组,对照组加50 mg/L的ox-LDL培养12小时;实验组分为2组,分别加50 mg/L的ox-LDL和不同浓度的阿托伐他汀培养12小时,于6小时和12小时进行检测,用ELISA法检测细胞培养上清液中TSLP的浓度,用RT-PCR检测TSLP mRNA的表达,采用免疫荧光检测细胞胞浆中TSLP表达.结果:ELISA和IF结果显示,实验组上清液和细胞浆内的TSLP的表达明显低于对照组,并随浓度的增大及时间的延长,TSLP降低得更明显.结论:阿托伐他汀可抑制ox-LDL诱导的HUVECs表达TSLP,这可能是阿托伐他汀抗动脉粥样硬化炎症反应的机制之一.
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