Objective:To investigate the specific immune act ivated effects in the mouse immunized by MHC I-restriced tumor antigen peptide pulsed dendritic cells(DC) with IL-2 gene modified.Methods:DC were transfected with IL-2 gene via adenovirus vector(DC-IL-2) the mRNA of mIL-2 was examined by RT-PCR.Syngeneic mice were immunized by DC-IL-2 pul sed with MHC-I-restricted Mutl tumor antigen peptiede of 3LL Lewis lung carcin oma (DC-IL-2-Mutl),the effects of lymphocyte subsets in the draining lymphoid node from the tumor antigen pulsed DCs with gene modified immunized mice by flo w-cytometric analysis(FACS) analysis.Results:The mRNA of mIL-2 was expressed in the DCs.The ratio of CD8+T cells was hosted in the dra ining lymphoid node from Mutl pulsed DCs(DC-Mutl) immunized mice.The ratio of C D8+T cells and NK cells were all hosted obviously in the draining lymphoid nod e from DC-IL-2-Mutl immunized mice .Conclusion:Immunized mice with DC-IL-2-Mutl can induce the mouse specific antitumor immunized affect in vivo and can activate a variety of immunized effect in the mouse.%目的：探讨MHC I类分子限制性肿瘤抗原多肽Mutl体外冲击白 细胞介素2(Interleukin-2，IL-2)基因修饰的树突状细胞(Dendrtic cells,DC)对小鼠体 内特异性免疫的活化机制。方法：用腺病毒作为载体介导小鼠IL-2基因修饰DC，用小鼠Lewis肺癌 3LL细胞株MHC I类分子限制性八肽Mutl冲击IL-2基因修饰的DC(DC-IL-2-Mutl)免疫小鼠 ，用流式细胞术(FACS)分析免疫保护小鼠接受3LL细胞再攻击后引流淋巴结内T细胞亚群的比 例变化。结果：用Mutl冲击的DC免疫的小鼠抵抗3LL细胞再攻击时，引流淋巴结 内CD8+T淋巴细胞比例明显升高，DC-IL-2-Mutl免疫保护的小鼠接受3LL细胞再攻击后 引流淋巴结内CD8+和NK细胞的比例都明显升高。结论：研究表明MHC I类分子限制性肿瘤抗原多肽体外冲击IL-2基因 修饰的DC免疫小鼠，能诱导小鼠产生特异性抗肿瘤免疫应答，并能活化小鼠体内多环节免疫功能。
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