目的 应用脉冲场凝胶电泳(PFGE)技术对某医院同一外科病房临床分离的8株肺炎克雷伯菌进行同源性分析,为医院感染控制提供依据.方法 对此8株肺炎克雷伯菌,采用微量肉汤稀释法和K-B法进行药物敏感试验,并以PFGE技术进行基因分型.结果 药物敏感试验结果显示,除菌株Kp1外,菌株Kp2等其他7株肺炎克雷伯菌对11种抗菌药物具有相同的药敏谱,且为多重耐药菌；PFGE分析亦显示此7株菌为同一谱型.结论此外科病房临床分离的8株肺炎克雷伯菌中有7株来源于同一克隆株,提示可能是一次医院感染暴发流行.%Objective To analyze 8 strains of Klebsiella pneumoniae isolated from the surgical ward in a hospital by pulsed-field gel electrophoresis(PFGE), so as to provide scientific evidence for the control of nosocomial infection. Methods Antimicrobial susceptibility test of Klebsiella pneumoniae was performed by micro-broth dilution and Kirby-Bauer method, genotyping was performed with PFGE. Results Antimicrobial susceptibility test showed that except the strain Kpl, 7 clinical strains were the same sensitivity to 11 antimicrobial agents, and they were all multi-drug resistant; PFGE showed that 7 strains were the same pattern. Conclusion Seven of 8 Klebsiella pneumoniae strains came from the same clone, which suggesting it might be an outbreak of nosocomial infectioa
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