本文建立了检测HBsAg/IgM复合物的ELISA技术,对各型乙型肝炎229例、甲型肝炎6例和非甲非乙型肝炎5例进行了检测,并以献血员100名和HBsAg阴性的其他疾病的患者60例作为对照.结果显示甲型肝炎、非甲非乙型肝炎,HBsAg阴性的其他疾病患者及献血员均为阴性;乙型肝炎患者中HBsAg携带者、急性肝炎、慢性迁延性肝炎、慢性活动性肝炎及肝硬化的检出率为41.7～50.5%,各组间无显著差异.HBsAg/IgM的存在与HBsAg、HBeAg、PHSAR及HBV-DNA密切相关.8份附标本用免疫电镜检查均找到HBV颗粒,提示HBsAg/IgM阳性反映HBV的复制并具有高度传染性.%Circulating HBsAg/IgM complexes were detected by ELISA in 229 eases of different clinical types of hepatitis B.Six cases of hepatitis A,5 of non-A non-B hepatitis,100 donors and 60 of HBsAg negative patients with other diseases served as controls.The results showed that this method is of higher precision.Its coefficients of variation were 1.8% to 7.8%.The reaction of positive samples was blocked by the addition of human IgM to the system,but not IgG nor PHSA.The positive rate showed no significant diffcrence among the patienis with various clinical types of HBV infection.HBsAg/IgM complexes were present frequently in the HBeAg and PHSAR positive samples,and closely correlated with the presence of HBV-DNA.The higher HBsAg titer,the more positive rate of HBsAg/IgM complexes.HBV particles were found in 8 positive samples by immunoelectron microscopy.
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