目的 观察风湿痹痛方对胶原诱导型关节炎(CIA)大鼠血清细胞因子及滑膜组织Fas/FasL mRNA表达的影响,探讨其相关的作用机制.方法 采用大鼠足部皮内注射牛Ⅱ型胶原乳化剂方法建立CIA模型.实验大鼠随机分为空白组、模型组、雷公藤多苷组和风湿痹痛方低、中、高各剂量组,各给药组给予相应药物灌胃,连续14 d.观察各组大鼠体质量和关节炎评分;ELISA检测血清白细胞介素(IL)-1β、IL-15、转化生长因子(TGF)-β1含量;RT-PCR检测滑膜组织Fas、FasL的mRNA表达.结果 与模型组比较,风湿痹痛方中、高剂量组大鼠足部关节炎评分和血清IL-15含量显著降低,TGF-β1含量显著升高(P<0.01),风湿痹痛方高剂量组Fas mRNA表达显著降低,FasL mRNA表达显著升高(P<0.05).结论 风湿痹痛方对CIA有一定的免疫调节作用,其机制可能是通过调控Fas/FasL凋亡系统,诱导滑膜组织细胞凋亡,抑制滑膜增生.%Objective To observe the effects of Fengshi Bitong Prescription (FSBT) on cytokines in serum and Fas/FasL mRNA expression system in synovium; To discuss relevant mechanism of action. Methods Rats were given foot intradermal injection of bovine type Ⅱ collagen emulsion to establish collagen-induced arthritis (CIA) model. After successful modeling, they were randomly divided into 6 groups: normal control group, CIA model group, tripterygium glycosides group, FSBT high-, medium-, and low-dose groups. Each medication group was given relevant medicine for gavage for 14 d. The weight and arthritis scores of CIA rats were observed. Serum levels of IL-1β, IL-15, and TGF-β1 were detected by ELISA. Expressions of Fas and FasL mRNA in synovium of CIA rats were detected by real-time PCR. Results Compared with model group, the foot arthritis score and serum levels of IL-15 in FSBT high- and medium-dose groups significantly decreased (P<0.01), and the serum level of TGF-β1 significantly increased (P<0.05). Expression of Fas significantly decreased and FasL significantly increased in FSBT high-dose group (P<0.05). Conclusion FSBT may have certain immune regulation effects on rheumatoid arthritis. Its mechanism may be in regulating Fas/FasL apoptosis system, thereby inducing apoptosis of synovial cells and inhibiting synovial hyperplasia.
展开▼
