Objective To investigate the effects of Xuanfeitongfufang Decoction on expressions of MD-2, NF-κB protein and its mRNA in lung of the rats with acute lung injury caused by LPS. Methods Wistar rats were randomly divided into normal group, model group, dexamethasone group and Xuanfeitongfufang Decoction large-, medium-, small-dose group, each group had eight rats. The ALI rat model was established by LPS tail-intravenous injection (6 mg/kg). The rats in Xuanfeitongfufang Decoction groups were pretreated by Xuanfeitongfufang Decoction (15.12, 7.56, 3.78 g/kg) for 3 days before LPS induced ALI. The rats in dexamethasone group were pretreated by dexamethasone (5 mg/kg). MD-2, NF-κB protein and its mRNA were measured by immunohistochemistry and PCR. The histopathology of the lung injury was observed by light microscope. Results Compared with normal group, the expression of MD-2, NF-κB protein and its mRNA were obviously increased in model group (P<0.01). Compared with model group, the expression of MD-2, NF-κB protein and its mRNA were obviously decreased in Xuanfeitongfufang Decoction groups and dexamethasone group (P<0.01), and there was no obvious difference between Xuanfeitongfufang Decoction groups and dexamethasone group. Light microscope observation indicates that there were large areas of pulmonary hemorrhage and necrosis in model group. While in Xuanfeitongfufang Decoction group and dexamethasone group, the pathological manifestations were much more ameliorated than those of the model group. The lung bronchiale inflammation appeared occasionally, and the edema was lightly. Conclusion Xuanfeitongfufang Decoction can lessen the injury of lung tissue and has protective effects on rats with ALI, the mechanism is possibly related to the inhibition of the expressions of MD-2 and NF-κB protein and its mRNA in injured lung tissues.%目的：观察宣肺通腑方对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠肺组织髓样分化蛋白-2(MD-2)、核因子-κB(NF-κB)蛋白及其基因表达的影响,并探讨其作用机制。方法 Wistar大鼠随机分为正常组、模型组、地塞米松组和宣肺通腑方大、中、小剂量组,每组8只。尾静脉注射LPS 6 mg/kg制备大鼠ALI模型。宣肺通腑方各组于造模前以15.12、7.56、3.78 g原药材/kg宣肺通腑方水煎液灌胃,1次/d,连续3 d；地塞米松组在造模前1 h按5 mg/kg腹腔注射地塞米松。造模后9 h麻醉取材,采用免疫组化ABC法和实时荧光定量聚合酶链反应检测MD-2、NF-κB蛋白及其基因表达,光镜下观察大鼠肺组织病理变化。结果与正常组比较,模型组MD-2、NF-κB蛋白及其基因表达均明显上调(P＜0.05,P＜0.01)；与模型组比较,地塞米松组和宣肺通腑方各组MD-2、NF-κB蛋白及其基因表达明显降低(P＜0.05,P＜0.01)；地塞米松组和宣肺通腑方各剂量组比较差异无统计学意义(P＞0.05)。病理观察结果显示,与正常组比较,模型组大鼠肺组织出现大片出血及坏死,炎症细胞大量浸润；宣肺通腑方各剂量组和地塞米松组大鼠肺组织病理改变明显轻于模型组,肺细支气管偶见炎症改变,水肿较轻,炎性细胞渗出较少。结论宣肺通腑方能减轻ALI大鼠肺组织损伤,对肺损伤有保护作用,其机制可能与其降低ALI大鼠MD-2、NF-κB蛋白及其基因表达有关。
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