Objective To develop an UPLC method for the fingerprints and simultaneous determination of rutin, forsythoside A and phillyrin in different processing method, different parts, different collecting place and time of the leaves of Forsythia suspensa. Methods The chromatographic separation was performed on a C18 column (2.1 mm× 100 mm, 1.7 μm), with gradient elution of methanol and 0.2% aqueous formic acid at a flow rate of 0.4 mL/min. The column temperature was set at 40 ℃. Results UPLC fingerprint was established with 15 common peaks and 3 peaks were identified. Rutin, forsythoside A and phillyrin showed good linearity (r2>0.999 7) within the test ranges. The average recoveries were 98.4%, 97.2%, 98.6%, respectively. Conclusion The method was simple, rapid, specific and reliable, which is useful for quality control for the leaves of Forsythia suspense.%目的 建立不同加工方法、不同部位、不同采集地点和时间的连翘叶超高液相色谱指纹图谱及主要成分芦丁、连翘酯苷A 和连翘苷含量测定方法.方法 色谱柱:Acquity UPLC BEH C18(1.7 μm,2.1 mm×100 mm);柱温:40 ℃;流动相:乙腈-0.2%甲酸溶液,梯度洗脱;流速:0.4 mL/min.结果 建立了连翘叶的UPLC 指纹图谱,共标定了15 个共有峰,指认了其中的3 个共有峰,并对指认的3 个主要成分芦丁、连翘酯苷A、连翘苷进行了定量检测,在检测的范围内线性关系良好,r 2 均大于0.999 7,平均回收率分别为98.4%、97.2%、98.6%.结论 此方法简便、准确、快速,为全面评价连翘叶的质量提供了可靠的分析方法.
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