Objective To study the adsorption and separation of licorice flavonoid with macroporous resins. Methods Eight types of macroporous resin were selected to compare their performances in absorbing and desorbing licorice flavonoid. The optimal type for licorice flavonoid was decided, meanwhile, its kinetic curve and dynamic absorbing behavior were studied. Results HPD300 resin possessed higher adsorption and desorption capacity. The appropriate adsorption and desorption conditions were as follows:concentration of sample was 2.0 mg/mL, velocity of sample solution was 1.5 BV/h, volume of sample solution was 2 BV (bed volume);velocity of 80%ethanol was taken as eluant 1.5 BV/h, and the volume was 3 BV. Flavonoid content was increased more than 2 times under above conditions. Conclusion HPD300 macroporous resin showed better comprehensive adsorption property. It can be used to purify and separate licorice flavonoid.% 目的研究大孔树脂对甘草黄酮吸附分离特性。方法选择8种大孔吸附树脂,比较其对甘草黄酮的吸附率和解吸率,筛选出最佳种类,并对其动力学曲线和动态吸附性能进行考察。结果 HPD300树脂对甘草黄酮有较好的吸附和解吸效果。其吸附分离甘草黄酮适宜的条件为：上样液浓度2.0 mg/mL,上样流速1.5 BV/h,上样量为2 BV(树脂床体积)；以80%乙醇洗脱,洗脱速率1.5 BV/h,洗脱剂用量为3 BV。用优化出的条件进行甘草黄酮的纯化,得到的黄酮纯度比纯化前提高2倍以上。结论 HPD300树脂综合性能较好,适合于甘草黄酮的分离纯化。
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