Objective To establish an HPLC method for the content determination of morroniside, sweroside, paeoniflorin and loganin of Liuwei Dihuang Decoction and its Cornus Officinalis-Cortex Moutan couple; To discuss the relationship between the whole prescription and the couple of main pharmacodynamic components. Methods The HPLC method was used at Hypersile C18 column (4.6 mm × 250 mm, 5 μm); the mobile phase consisted of methanol-water (24:76); the detective wavelength was 236 nm; the flow rate was 1.0 mL/min; the column temperature was 30 ℃. Results The linear ranges of morroniside, sweroside, paeoniflorin and loganin were among 0.480–7.680 μg (r=0.999 3), 0.103–1.650 μg (r=0.999 5), 0.120–1.920 μg (r=0.999 1) and 0.227–3.630 μg (r=0.999 7), respectively. The average recovery rates and RSD were 102.79%, 102.29%, 100.99%, 102.48%, and 1.73%, 1.48%, 1.32%, 0.75%, respectively. The contents of morroniside, sweroside and paeoniflorin in Liuwei Dihuang Decoction were slightly higher than that in Cornus Officinalis - Cortex Moutan couple, and the contents of loganin were almost the same. Conclusion The method is simple, stable, accurate and reproducible. It can be used for content determinate of glycosides in Liuwei Dihuang Decoction and Cornus Officinalis-Cortex Moutan couple. Cornus Officinalis-Cortex Moutan couple has the glycosides with tonifying kidney effect of Liuwei Dihuang Decoction.%目的 建立HPLC同时测定六味地黄汤及方中山茱萸-牡丹皮药对莫诺苷、獐芽菜苷、芍药苷、马钱苷含量的方法,探讨全方与该药对主要药效成分之间的关联性.方法 采用Hypersil C18色谱柱(4.6 mm× 250 mm,5 μm),流动相为甲醇-水(24∶76),流速1.0 mL/min,检测波长236 nm,柱温30 ℃.结果 莫诺苷、獐牙菜苷、芍药苷和马钱苷分别在0.480～7.680 μg(r＝0.999 3)、0.103～1.650 μg(r＝0.999 5)、0.120～1.920 μg(r＝0.999 1)、0.227～3.630 μg(r＝0.999 7)范围内呈良好的线性关系,平均回收率分别为102.79%、102.29%、100.99%、102.48%,RSD分别为1.73%、1.48%、1.32%、0.75%.六味地黄汤中莫诺苷、獐芽菜苷、芍药苷含量略高于山茱萸-牡丹皮药对,马钱苷含量基本相同.结论 本方法简便可靠、重复性好,可用于六味地黄汤及山茱萸-牡丹皮药对苷类成分含量测定.山茱萸-牡丹皮药对含有六味地黄汤补肾作用的核心苷类成分.
展开▼
