Two fluorescent probes BMO and NBMO were developed for the highly selective detection of Zn2+ based on C=N isomerization and chelation-enhanced fluorescence (CHEF) mechanism.Structure identification of the probes was confirmed by 1H NMR,13C NMR,1H-1H COSY,HSQC,IR and HRMS spectroscopy.The results of spectral analysis show that BMO and NBMO are sensitive and highly selective to Zn2+.The detection limit of BMO and NBMO are found to be 30 and 21 nmol·L-1 respectively.There is a good linear relationship between the fluorescence intensity of probes (BMO and NBMO) and the concentration of Zn2+ in the range of 0 to 20 μmol· L-1.The X-ray crystal structure of the NBMO-Zn2+ complex exposes its coordination feature and the Job plots reveal a 1∶1 probe-Zn2+ identification.The cell images show that NBMO can be used to detect intracellular Zn2+.%设计合成了1种基于C=N异构化和螯合荧光增强机理(CHEF)的Zn2+荧光探针BMO和NBMO,其结构经1H NMR,13CNMR,1H-1H COSY,HSQC,IR和HRMS进行了表征.光谱分析实验结果显示,探针对Zn2+均具有较好的选择性和灵敏度,检出限分别为30和21 nmol·L-1.在0～20 μmol·L-1浓度的范围内,BMO和NBMO的荧光强度与Zn2+浓度可呈良好的线性关系.NBMO-Zn2+配合物单晶结构和Job曲线证实该探针与Zn2+以1∶1配位.NBMO被成功应用于活细胞中Zn2+的检测.
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