目的:探讨移植肾组织中整合素连接激酶(ILK)及Ⅳ型胶原的表达,及与转化生长因子-β1(TGF-β1)表达和慢性移植肾肾病(CAN)的关系.方法:用免疫组织化学技术和计算机真彩色图像分析系统半定量检测30例CAN患者移植肾组织中ILK、Ⅳ型胶原和TGF-β1的表达情况,分析三者间及与CAN病理分级之间的关系.10例正常肾组织作为对照.结果:CAN移植肾组织中ILK、Ⅳ型胶原、TGF-β1的表达比正常肾组织明显增加(P<0.001),并随CAN病理分级呈逐渐递增的趋势.移植肾组织中ILK的表达与TGF-β1、Ⅳ型胶原呈正相关(r分别为0,822、0,715,P<0,001).结论:研究结果显示ILK可能介导TGF-β1促进CAN细胞外基质(ECM)异常沉积发病机制,Ⅳ型胶原的异常沉积是CAN患者移植肾纤维化的重要表现,且ILK在CAN移植肾纤维化进程中起重要作用.%Objective:To investigate the expressions of integrin- linked kinase(ILK) and collagen Ⅳ in the renal allografts of patients with chronic aUograft nephropathy (CAN), and explore their relationship with transforming growth factor-β1(TGF-β1) ex-pression and the pathogenesis of CAN. Methods: Immunohistochemical assay and computer- assisted genuine colored image analysis system were used to detect the expressions of ILK,TGF-β1and collagen Ⅳ in the renal aUografts of patients with CAN. The asso-ciation between TGF- β1 ,collagen Ⅳ and ILK,as well as the relationship between their expressions and the pathological grading of CAN,were analyzed. Ten specimens of healthy renal tissue were used as controls. Results: The expressions levels of ILK, TGF - β1and collagen Ⅳ were significantly higher in the renal tissues of the patients, compared to those in the normal renal tissues(P<0.001) ,and the expressions tended to increase with the pathological grades of CAN. In CAN group, tbe expression of ILK was posi-tively correlated with those of TGF - β1 and collagen IV ( r = 0. 822 and r = 0.715, respectively; P < 0.001 for both ). Conclusion: It is indicated by the data that ILK might mediate the mechanism through which TGF- β1 promoted the abnormal deposition of ECMin renal aUograft with CAN and the abnormal deposition of collagen Ⅳ in renal aUngraft might be important manifestation of fibrosis in CAN. It is suggested that ILK might play an important role in the progression of renal fibrosis in CAN.
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