目的 探讨超顺磁性纳米氧化铁颗粒(SPION)靶向标记胰腺癌细胞(BxP&3)行MR分子成像的可行性.方法 制备黏蛋白1(MUC1)靶向修饰的探针MUC1 SPION(靶向组),并以牛血清蛋白(BSA)制备非靶向探针BSA-SPION(非靶向组).采过噻唑蓝(MTT)法测试不同MUC1-SPION浓度下(铁浓度为0、6.25、12.50、25、50、100、200 μg/ml)的细胞毒性.并分别于铁浓度为50、100、200 μg/ml条件下,对靶向组及对照组与BxPC-3细胞共同孵育2h后的细胞悬液进行MR成像,测定横向弛豫时间(T2),计算T2强化率.以普鲁士蓝染色观察靶向探针与细胞结合情况.结果 MTT法细胞毒性实验显示,不同MUC1-SPION浓度下BXPC 3细胞增殖率差异无统计学意义(F=1.74,P=0.183).铁浓度50、100、200 μg/ml条件下,2组间T2值和T2强化率的差异均有统计学意义(P均＜0.05).普鲁士蓝染色显示靶向组的蓝染颗粒更多.结论 MUC1-SPION对BxPC-3细胞具有良好的靶向性,以SPION靶向标记BxPC-3细胞进行MRI安全、可行.%Objective To explore the feasibility of MR molecular imaging in human pancreatic cancer cell (BxPC-3 cell) targeted by superparamagnetic iron oxide nanoparticles (SPION).Methods Both MUC1 SPION probes with MUC1 targeted modification (targeted group) and bull serum albumin (BSA)-SPION as the control (non targeted group) were prepared.The cytotoxicity of MUC1 SPION in different concentrations (0,6.25,12.50,25,50,100,200 μg/ml) was verified by MTT (3 [4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay.BxPC-3 cells were cultured with MUC1-SPION (targeting group) and BSA-SPION (control group) in different concentration as 50,100,200 μg/ml,respectively for 2 h.Then MRI scans were performed,the transverse relaxation time (T2) value and the enhancement ratio of T2 were recorded and calculated.The combination conditions of targeting probes and cells were observed by prussian blue staining.Results The cell cytotoxicity of MUC1 SPION in different concentrations showed no statistical difference according to MTT assay (F=1.74,P 0.18).There were statistical differences of the T2 value and the enhancement ratio of T2 for the concentration as 50,100,200 μg/ml,respectively (all P＜0.05).More blue particles were observed by prussian blue staining in targeted group than in non targeted group.Conclusion MUC1-SPION has favourable targeting ability to BxPC 3 cell,and MRI of BxPC-3 cell targeted by SPION is satisfied security and feasibility.
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