目的 制备人卵巢癌靶向超声造影剂,观察其体外寻靶能力.方法 采用生物素-链霉亲和素法制备促黄体生成素释放激素类似物(LHRHa)靶向脂质微泡,以免疫荧光染色验证LHRHa与脂质微泡的结合情况,并以普通脂质微泡作为对照,在倒置显微镜下观察LHRHa靶向脂质微泡对人卵巢癌A2780/DDP的体外寻靶能力.结果 LHRHa靶向脂质微泡免疫荧光阳性;体外寻靶实验显示LHRHa靶向脂质微泡能够与表面存在LHRH受体的A2780/DDP细胞特异性结合,而普通脂质微泡则不能结合.结论 利用生物素-链霉亲和素方法可成功制备LHRHa靶向脂质微泡造影剂,该靶向造影剂具有体外寻靶能力.%Objective To prepare targeted microbubbles targeting ovarian ovarain cells , and to assess its targeting ability in vitro . Methods The biotinylated luteinizing hormone releasing hormone analog (LHRHa) peptides were conjugated to the bio tinylated microbuhhles via streptavidin , and then were observed with fluo rescence microscope . The LHRHa carrying microbuibbles were added into A2780/DDP cells to observe the adherence of the targeted microhubbles to the cells , and normal microbubbles were used as control. Results Fluorescence was expressed on the targeted microbubhles shell in vitro . The LHRHa carrying microbubles could adhere to the A 2780/DDP cells specifically , whereas there was no adherence of normal microbubbles to the A2780/DDP cells . Conclusion The LHRHa carrying microbubbles having targeting ability in vitro can be successfully constructed via biotin-sterptavdin binding .
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