纳秒脉冲电场(Nanosecond Pulsed Electric Fields, nsPEFs)是一种有望应用于临床肿瘤治疗的新兴方法.前期研究表明,nsPEFs可抑制人黑色素瘤细胞A375的细胞活性和迁移,但与抗肿瘤药物联用是否有协同作用有待研究.本文采用CCK-8法考察nsPEFs与抗肿瘤药物达卡巴嗪和顺铂联合作用对A375细胞活力的影响.结果发现,nsPEFs联合达卡巴嗪协同作用不显著,但与顺铂联合作用则具有强协同作用,且与nsPEFs的输入能量正相关.该研究结果可为nsPEFs联合抗肿瘤药物更好应用于临床提供实验依据.%Nanosecond pulsed electric fields (nsPEFs) are promising in the treatment for cancer in clinic. Our previous study proved that nsPEFs can inhibit the cell viability and migration of human melanoma cell line A375. However, whether there is a synergy between nsPEFs and anti-tumor drugs remains unknown. Herein CCK-8 assay is used to investigate the synergistic effect of nsPEFs and anti-tumor drugs, dacarbazine and cisplatin, on the cell viability of A375 cells. The results show that nsPEFs combined with dacarbazine has a trivial synergistic effect, while nsPEFs combined with cisplatin has significant synergistic effect which is positively correlated with the input power of nsPEFs. This study provides research data for the application of nsPEFs combined with anti-tumor drugs in clinic.
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