Objective:To investigate the toxic effects of clenbuterol hydrochloride (CL)on cardiomyocytes (CMs)derived from human induced pluripotent stem cells (hiPSCs).Method:Pluripotent stem cells derived from human skin fibroblasts were directly differentiated into CMs.The iPSC-derived CMs (iPSC-CMs)were then ran-domly divided into 6 groups and treated with different concentrations of CL.The cellular structure,beating frequen-cy and apoptosis rates of these iPSC-CMs were evaluated in groups.Results:After CL treatment,iPSC-CMs mor-phologically exhibited a smaller size.Under 50-100μg/ml of CL,myofibers of the iPSC-CMs seemed irregular and exhibited a granular morphology.The beating frequency of iPSC-CMs and CL concentration were positively correla-ted.Compared with the control,the apoptosis rates in iPSC-CMs treated with 10μg/ml and group 50μg/ml were significantly increased.Conclusion:CL has toxic effects on human cardiomyocytes derived from hiPSCs.%目的：：探讨盐酸克伦特罗对人类诱导多能干细胞(iPSC)来源心肌细胞的毒性作用。方法：将人皮肤来源的 iPSC 成功定向分化为心肌细胞,加入不同浓度盐酸克伦特罗,使之分为对照组(0μg/ml)、1μg/ml 组、10μg/ml 组、20μg/ml 组、50μg/ml 组、100μg/ml 组等6组(n 均=3),相同条件共培养7天后,观察盐酸克伦特罗对心肌细胞形态大小、搏动频率及凋亡率的影响。结果：不同浓度盐酸克伦特罗均可使心肌细胞缩小(P <0.05),50-100μg/ml 盐酸克伦特罗可致心肌细胞骨架断裂,甚至呈颗粒状。各种浓度盐酸克伦特罗均致心肌细胞搏动频率加快(P <0.05),且有浓度越高搏动越快的趋势。10μg/ml 组和50μg/ml 组所致心肌细胞凋亡率显著高于对照组(P <0.05)。结论：盐酸克伦特罗对人类皮肤来源 iPSC 分化的心肌细胞有毒性作用。
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