目的 观察吗啡自身给药模型大鼠复燃后药物戒断的行为学表现,评估大鼠自主觅药后的抑郁行为并探讨自发性觅药大鼠戒断后抑郁模型的建立.方法 16只大鼠随机分为对照组和模型组(n ＝8).两组大鼠均行右侧颈静脉置管术.模型组采用自身给药(self-administration)建立吗啡成瘾模型,复燃戒断后运用改良的Gellert-Hohzman法进行药物戒断症状的评价,分别用大脑系数评价,旷场实验(open field test),糖水消耗实验,食物消耗量及体重变化,评估吗啡戒断后第3d大鼠的行为学表现.结果 在自身给药大鼠在复燃后的戒断期里,饮食量和体重降低,在复燃后戒断的第3d,大脑系数降低,旷场试验评分,糖水消耗量,1％蔗糖偏爱百分比(P＜0.05)较正常对照组均降低.结论 自身给药模型大鼠在复燃后戒断期中戒断症状明显并导致抑郁行为,与人类的成瘾复吸行为有良好的相似性,可做为戒断后抑郁实验模型并为戒断后抑郁的治疗提供实验基础.%Objective To observe the behaviors of drug self-administration rats after morphine withdrawal,evaluate the depressive-like behaviors after drug withdrawal and discuss the establishment of depression rat model.Methods A total of 16 SD rats were randomly divided into drug self-administration group (model group) and saline treatment group (NS group).The chronic intravenous catheter was conducted for all 16 rats.Morphine selfadministration model was established in model group (8 rats),and then all rats relapsed.Their behaviors were evaluated with Gellert-Hohzman method at 3 d after withdrawal in the following tests:open field test,sucrose preference test,the test of body weight and diet amount changes and the coefficient of brain.Results In the period of withdrawal after relapse,body weight and diet amount,the coefficient of brain,open field tests scores,sucrose consumption and the sucrose preference were decreased (P ＜ 0.05).Conclusion In the period of withdrawal after relapse,withdrawal symptoms of morphine self-administration rats which lead to the depressive-like behaviors are significant.In view of the similarity with human addiction relapse behavior,drug self-administration model can be used as depression experimental model.
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