目的 探讨协同刺激分子CD86在母-胎免疫调节中的作用。方法 建立自然流产模型（CBA×DBA/2）及正常妊娠模型（CBA×BALB/c）。两种模型分别再分为3个组：（1）以大鼠的IgG为对照的对照组；（2）于妊娠第4、6、8、10天，分别给CBA孕鼠腹腔注射大鼠抗小鼠CD86单克隆抗体的多次干预组；（3）仅于妊娠第4天，给CBA孕鼠单次腹腔注射抗CD86单克隆抗体的单次干预组。每次腹腔注射的抗体剂量均为100 μg。于妊娠第14天计算各组胚胎吸收率。结果 （1）自然流产模型与正常妊娠模型的对照组胚胎吸收率分别为27.78%和8.42%。（2）于妊娠第4、6、8、10天腹腔注射抗CD86单克隆抗体后，自然流产模型的多次干预组胚胎吸收率下降至9.68%（P<0.05）；而正常妊娠模型的多次干预组胚胎吸收率上升至13.54%（P>0.05）。（3）妊娠第4天单次腹腔注射抗CD86单克隆抗体，自然流产模型单次干预组的胚胎吸收率下降至7.14%（P<0.001）；而正常妊娠模型单次干预组的胚胎吸收率上升至11.39%（P>0.05）。结论 妊娠早期，尤其在胚胎对母体的致敏阶段（着床期），阻断母-胎界面的CD86协同刺激信号，将诱导母体对胚胎的免疫耐受，从而减少胚胎吸收，提高妊娠成功率。%Objective To evaluate the role of costimulating molecule CD86 in maternal-fetal immune regulation. Methods In abortion-prone (CBA×DBA/2) matings and non-abortion-prone (CBA×BALB/c) matings, the pregnant CBA females were injected intraperitoneally by purified rat anti-murine CD86 monoclonal antibody (mAb) with dosage of 100 μg at day 4, 6, 8 and 10 of gestation respectively, or injected only once at day 4 of gestation, or injected with purified rat IgG as control. The resorbing and non-resorbing embryos were enumerated at day 14 of gestation. Results The embryo resorbing rate in CBA×DBA/2 matings was 27.78%, and that in CBA×BALB/c matings was 8.42%. After injection of anti-CD86 mAb at day 4, 6, 8, 10 of gestation, the embryo resorbing rate reduced to 9.68% (P<0.005) in CBA×DBA/2 matings, while that was 13.54% in CBA×BALB/c matings (P>0.05). After injection of anti-CD86 mAb only once at day 4 of gestation, the embryo resorbing rate reduced apparently to 7.14% (P<0.001) in CBA×DBA/2 matings, while that was 11.39% (P>0.05) in CBA×BALB/c matings. Conclusions Blocking the costimulating signal by anti-CD86 mAb at maternal-fetal interface, especially at the prime stage of maternal-fetal recognition (periods of implantation), may result in maternal-fetal tolerance, and hopefully the embryo resorption turns to normal pregnancy.
展开▼
