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首页> 外文期刊>中国海洋湖沼学报（英文版） >The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri
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The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri

机译：等酶与RAPD标记物比较对扇贝衣藻种群遗传结构的分析。

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摘要

To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamys farreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-PP, ACP, AK, PK, AAT, SOD, EST) in three buffer systems (TC, pH6.9; TMME, pH 7.4; and EBT, pH8.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep-LG-1, Pep-PP, Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.
机译：为了比较遗传标记以进行群体遗传分析，采用了同工酶电泳和随机扩增多态性DNA（RAPD）来检测扇贝衣原体种群的遗传结构。在三个缓冲液系统（TC，pH6.9; TMME，pH 7.4）中的十三种酶（MDH，ME，IDH，GPI，PGM，PEP-LG，PEP-PP，ACP，AK，PK，AAT，SOD，EST）选择EBT，pH8.9）并使用22个基因座进行分析，其中7个基因座（Gpi，Pgm，Pep-LG-1，Pep-PP，Aat-2，Est-2，Est-3）是多态的占总数的31.82％。杂合子的平均值为0.113，大多数研究的基因座显示杂合子缺陷。使用10个任意选择的引物（10个碱基）研究了相同的标本。 54个RAPD片段中有22个为多态性，平均杂合度为0.194。结果表明，两种类型的标记物反映了群体遗传多样性参数值的一致趋势，但是RAPD揭示的遗传变异信息比同工酶电泳更多。

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