Purification and characterization of lectin from humoral fluids of Charybdis feriatus

DAI Congjie; Wang Guizhong; HE Jianfeng; LI Shaojing; HUANG Huiyang

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Purification and characterization of lectin from humoral fluids of Charybdis feriatus

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To search new sources of lectin, an experiment on lectin distribution in humoral serum of crab Charybdisferiatus (in short, CFL) was conducted March, 2002. When adding solid ammonium sulfate into the fluids up to 50% saturation at 4℃, most CFL activity showed precipitates who were then continually extracted by ammonium sulfate of different concentrations. The supernatant, which was called primary CFL fluids, was given a 17.60-fold purification and 45.70% recovery of total activity. Finally, by using Sephadex G-100 column chromatography, the CFL in the primary CFL fluid was highly purified.Compared to the original humoral fluids, the last purified CFL got a 203.90-fold purification and 30.48%recovery of total activity, and demonstrated a single band on SDS-PAGE. In thesame time, the purified CFL was detected for agglutination activity with 7 kinds of animal erythrocytes. Other characterization,such as sugar inhibition, and the effect of temperature on the agglutination activity of CFL were also studied. Our results indicate that agglutination activity of CFL was influenced by sugar and temperature.

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《中国海洋湖沼学报（英文版）》 |2006年第4期|390-394|共5页
作者
DAI Congjie; Wang Guizhong; HE Jianfeng; LI Shaojing; HUANG Huiyang;
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作者单位

Department of Oceanography, State key Lab of Marine Environmental Sciences, Xiamen University, Xiamen 361005, China;

Polar Research Institute of China, Shanghai 200136, China;

Department of Biology Quanzhou Normal College, Quanzhou 362000, China;

Department of Oceanography, State key Lab of Marine Environmental Sciences, Xiamen University, Xiamen 361005, China;

Polar Research Institute of China, Shanghai 200136, China;

Department of Oceanography, State key Lab of Marine Environmental Sciences, Xiamen University, Xiamen 361005, China;

Department of Oceanography, State key Lab of Marine Environmental Sciences, Xiamen University, Xiamen 361005, China;

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正文语种 chi
中图分类海洋学;
关键词
ammonium sulfate; purification; CFL (lectin from Charybdisferiatus); characterization;

机译：硫酸铵;纯化;CFL（Charybdisferiatus的凝集素）;表征;

Purification and characterization of lectin from humoral fluids of Charybdis feriatus

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