Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

YOU Feng; ZHANG Peijun; WANG Keling; XIANG Jianhai

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Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

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Population genetics of the left-eyed flounder, Paralichthys olivaceus, including natural and cultured stocks distributed in the coastal waters near Qingdao of eastern maritime China, was analyzed in allozyme and RAPD. The results showed that among total 29 gene loci of 15 isozymes, 9 and 7 were polymorphic in natural and cultured stocks, respectively. The status of genetic diversity in P olivaceus is low in terms of polymorphic loci in chi-square test and genetic departure index of Hardy-Weinberg equilibrium. More alleles in IDHP, CAT, GDH and Ldh-C allozymes were found in the fish, which could be used as markers in assortive breeding and distinguishing stock, population or species evolution. Total 88 and 86 RAPD bands ranging from 200 to 2 500 bp were recognized individually in average of 7.8-8.0 bands per primer. The genetic diversity in cultured stock is lower than that in natural ones showing an obviously decreasing genetic divergence. Therefore, effective countermeasures must be taken to protect genetic resources of marine cultured fishes. The 2 markers have their own pros and cons. Combining the 2 markers to investigate the genetic variation of populations is suggested. The results provide basic data of this flounder and they are useful for studying genetic improvement and genetic resources of the fish.

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《中国海洋湖沼学报（英文版）》 |2007年第1期|78-84|共7页
作者
YOU Feng; ZHANG Peijun; WANG Keling; XIANG Jianhai;
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作者单位

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;

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原文格式 PDF
正文语种 chi
中图分类地球物理学;
关键词
Paralichthys olivaceus; genetic variation; natural and cultured stocks; allozyme; RAPD;

机译：副寄生;遗传变异;自然和养殖种群;酶;RAPD;

Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

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