首页> 中文期刊> 《中国海洋湖沼学报(英文版)》 >Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus

Polyploidy induction by hydrostatic pressure shock and embryo development of sea cucumber Apostichopus japonicus

         

摘要

The manipulation of the chromosome set for commercially valuable marine animals is important for enhancing aquacultural production. In this study, triploid and tetraploid sea cucumber Apostichopus japonicus were induced by hydrostatic pressure shock, and the conditions of appropriate induction were tested with different starting times, and hydrostatic pressure intensities and durations. The highest rate of triploid induction reached 20% and that of tetraploid was 60%. In consideration of the survival rate and hatch rate, the appropriate treatment for triploid was 55 Mpa of hydrostatic pressure for 5 min at 55 min after fertilization (a.f.), while for tetraploid it was 60 Mpa for 5 min at 61 min a.f. The triploid of the sea cucumber could survive through the pelagic larval stage and attachment stage, and develop like the control group of the experiment. The tetraploid, however, could not survive the attachment stage.

著录项

  • 来源
    《中国海洋湖沼学报(英文版)》 |2007年第2期|184-190|共7页
  • 作者单位

    School ofEnvironmental & Biological Science & Technology,Dalian University Technology,Dalian 116024,China;

    Key Laboratory of Mariculture and Biotechnology,Ministry of Agriculture,Dalian Fisheries University,Dalian 116023,China;

    Key Laboratory of Mariculture and Biotechnology,Ministry of Agriculture,Dalian Fisheries University,Dalian 116023,China;

    Key Laboratory of Mariculture and Biotechnology,Ministry of Agriculture,Dalian Fisheries University,Dalian 116023,China;

    Key Laboratory of Mariculture and Biotechnology,Ministry of Agriculture,Dalian Fisheries University,Dalian 116023,China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 动物学;
  • 关键词

    triploid; tetraploid; hydrostatic pressure shock; sea cucumber; Apostichopus japonicus;

    机译:三倍体;四倍体;静水压​​休克;海参;刺参;
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