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首页> 外文期刊>中国海洋湖沼学报（英文版） >In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus
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In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

机译：新烟碱类杀虫剂吡虫啉对比目鱼副蝇g细胞系的体外急性细胞毒性

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摘要

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR, MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.
机译：通过3种广泛使用的终点生物测定法检测了新烟碱类杀虫剂吡虫啉（IMI）对收集在比目鱼g中的比目鱼（FG）ill细胞系的体外急性细胞毒性：NR（中性红），MTT（3-（ 4,5-二甲基噻唑-2-基）-2、5-二苯基溴化四唑）和TCP（总细胞蛋白）。结果表明，浓度≥0.5μg/ ml时，IMI升高。 NR，MTT和TCP的IC50值分别为41.86、38.46和39.08μg/ ml。超微结构观察表明，暴露于60μg/ ml IMI 48 h的细胞线粒体受到严重破坏，肿胀或破坏，而其细胞核和粗面内质网（RER）保持正常。这表明线粒体可能是IMI的主要靶标。

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