Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

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Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

机译：蛤lam肌动蛋白基因的克隆，鉴定及软体动物肌动蛋白的系统发育分析

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摘要

An actin gene (CfACT1) was cloned by using RT-PCR, 3' and 5'RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1535 bp, which contains a long 3' un-translated region of 436bp and 59bp of a 5' un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACT1 is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic, although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACT1 was cloned and sequenced. Only one intron was detected:it was located between codons 42 and 43 and different from vertebrate actin genes.

机译：通过使用RT-PCR，海扇贝衣藻衣藻血细胞的3'和5'RACE克隆了肌动蛋白基因（CfACT1）。转录物的全长为1535bp，其包含436bp的长3'非翻译区和5'非翻译序列的59bp。开放阅读框编码376个氨基酸的多肽。序列比较表明，CfACT1与脊椎动物细胞质肌动蛋白的关系比肌肉类型更为紧密。系统发育分析表明，软体动物肌动蛋白一般可分为两类：肌肉和细胞质，尽管两者都与脊椎动物的细胞质肌动蛋白相似。还可以推断，软体动物的肌肉或细胞质中存在不同的同种型。克隆并测序了CfACT1的基因组序列。仅检测到一个内含子：它位于第42和43号密码子之间，不同于脊椎动物的肌动蛋白基因。

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《中国海洋湖沼学报（英文版）》 |2007年第3期|304-309|共6页
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作者单位

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;

Life Science and Technology Division, Ocean University of China, Qingdao 266003, China;

Life Science and Technology Division, Ocean University of China, Qingdao 266003, China;

Life Science and Technology Division, Ocean University of China, Qingdao 266003, China;

Life Science and Technology Division, Ocean University of China, Qingdao 266003, China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类动物学;
关键词
Chlamys farreri; actin; clone; phylogenetic analysis; intron;

机译：花re;肌动蛋白;克隆;系统发育分析;内含子;

Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

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