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《中国油料作物学报（英文）》
>Identification of differentially expressed genes between Sclerotinia-resistant and its near-isogenic susceptible lines of Brassica napus infected with Sclerotinia sclerotiorum
Identification of differentially expressed genes between Sclerotinia-resistant and its near-isogenic susceptible lines of Brassica napus infected with Sclerotinia sclerotiorum
The gene-expression profiles between the Sclerotinia-resistant and its near-isogenic susceptible rapeseed lines (Brassica napus) infected with Sclerotinia sclerotio-rum were examined by microarray analysis. 102 differentially expressed genes (DEGs) were successfully identified, including genes highly related to pathogen resistance. Among these DEGs, 46 were up-regulated and 56 were down-regulated. To verify the reliability of microarray, 2 up-regulated genes (NM_130355 and NM_126666) and 1 down-regulated gene (NM_100191) were chosen and their expression level were detected by real-time flu-orescence-based quantitative PCR analysis (RT-PCR). Subsequently, the 102 DEGs were GO (gene ontology) annotated, and to be classified into 22 categories, including enzymes, binding proteins, transcription factors, and proteins with unknown functions. Further sub-cellular localization prediction demonstrated that these DEGs might be located in/on intra-cellular region, cell wall, cell membrane, cytoplasm, nucleus, endoplasmic reticulum, chlo-roplasts and mitochondria. The analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that 10 DEGs were participated in several known biological pathways, including lysine biosynthesis, sulfur assimilation, cysteine metabolism, carot-enoid biosynthesis, glyoxylic acid and oxalic acid metabolism etc. Taken together, the pre-liminary results would provide important information for exploring the molecular basis of Sclerotinia-resistance in Brassica napus.
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