目的：探讨桔梗皂苷D对白色念珠菌黏附口腔黏膜上皮细胞的影响。方法： MTT法检测不同桔梗皂苷D对口腔上皮KB细胞存活率的影响；将白色念珠菌、KB细胞以及不同浓度的桔梗皂苷D共同培养，革兰染色检测白色念珠菌黏附数，台盼蓝排斥实验检测念珠菌活力和菌丝转换率；ELISA法检测上清液中IL－18与人β－防御素2（HBD－2）的蛋白含量；实时荧光定量PCR和Western blot 法分别检测KB细胞中HBD－2 mRNA与蛋白表达的变化。结果：桔梗皂苷D对KB细胞存活率无影响；随着桔梗皂苷D浓度的增加，白色念珠菌的黏附数、菌活力和菌丝转换率逐渐下降，上清液中IL－18与HBD－2的蛋白含量以及KB细胞中HBD－2 mRNA表达与蛋白水平逐渐降低。结论：桔梗皂苷D具有抑菌作用，并参与了口腔黏膜上皮细胞防御白色念珠菌感染的免疫反应。%AIM: To investigate the effect of platycodin D on Candida albicans infection in oral epithelial cells.METHODS:The viability of the oral squamous carcinoma KB cells was detected by MTT assay after treated with different concentrations of platycodin D .The KB cells were infected with Candida albicans, and then were incubated with platycodin D at different concentrations .Adherent numbers of the Candida albicans were counted by Gram staining , and the bacterial activity and conversion were measured by Trypan blue staining .Furthermore , the protein levels of IL-18 and hu-man β-defensin 2 ( HBD-2) were analyzed by ELISA , and the expression of HBD-2 at mRNA and protein levels was deter-mined by RT-qPCR and Western blot , respectively .RESULTS:The viability of the KB cells was not affected by platycod-in D at the concentrations used .The adherent numbers , bacterial activity and conversion were decreased by treatment with platycodin D in a dose-dependent manner .In addition, the protein level of IL-18 in the culture supernatant and the mRNA expression of HBD-2 in the KB cells were also reduced after platycodin D treatment .CONCLUSION:Platycodin D has a bacteriostasis effect and prevents oral epithelial cells from Candida albicans infection.
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