目的:探讨染料木黄酮对氨所致星形胶质细胞NF-κB活化的影响及其机制.方法:以氯化铵刺激原代培养的星形胶质细胞,建立高血氨模型,Western blot检测星形胶质细胞中ERK,Akt及核因子κB (NF-κB)的活化.结果:AG1478及染料木黄酮可显著抑制氨诱导的星形胶质细胞ERK及Akt的活化.LY294002、染料木黄酮及AG1478可显著抑制氨诱导的星形胶质细胞NF-κB核转位.结论:染料木黄酮可显著抑制氨诱导的星形胶质细胞ERK活化和Akt介导的NF-κB活化,这可能是该天然物质抑制星形胶质细胞水肿的重要机制.%AIM:To explore the effect of genistein on ammonia-induced nuclear factor-κB (NF-κB) activation and the underlying mechanism.METHODS:Primary astrocyte cultures were prepared and challenged with NH4Cl to establish a hyperammonemic model.The activation of ERK,Akt and NF-κB was examined by Western blot.RESULTS:AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt.Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002,genistein and AG1478.CONCLUSION:Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation,which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect.
展开▼
