AIM:To investigate the effects of ethane 1,2-dimethanesulfonate (EDS) preconditioning on renal ischemia/reperfusion (I/R) injury in male Sprague-Dawley (SD) rats.METHODS: Male SD rats (n=48) were ran-domly assigned to 6 groups:blank, sham, I/R, EDS+I/R, EDS+testosterone (TST) +I/R, and castration (Cast)+I/R.The renal pedicles were bilaterally occluded with a microvascular clamp for 45 min to establish renal I/R-induced in-jury model.Bilateral orchiectomy was conducted 2 weeks before surgery .EDS (75 mg/kg) was intraperitoneally injected 5 d before operation .Blood samples were collected 24 h after reperfusion from the vena orbitalis posterior plexus .Luteinizing hormone (LH), TST, serum creatinine (SCr), blood urea nitrogen (BUN), and kidney injury molecule-1 (KIM-1) were detected.The renal tissues were harvested to measure the level of TNF-αand the expression of Fas mRNA and caspase-3 protein.RESULTS:Serum TST levels in EDS +I/R group and Cast +I/R group were below the minimum detectable threshold.Compared with other groups , the rats in EDS+I/R group and Cast +I/R group had higher levels of SCr , BUN and KIM-1 (P<0.05).SCr and BUN levels showed no significant difference between EDS +I/R group and Cast +I/R group (P>0.05), but KIM-1 level in EDS+I/R group was lower than that in Cast +I/R group (P<0.05).After reper-fusion for 24 h, the levels of TST and LH in EDS +I/R group, Cast+I/R group and EDS+TST+I/R group were lower than those 1 h before operation (P<0.05).Compared with Cast+I/R and I/R group, the TNF-αlevel and expression of Fas mRNA and caspase-3 protein were significantly decreased in EDS +I/R group ( P<0.05 ) .CONCLUSION: EDS preconditioning substantially reduces the serum TST level , thus attenuating I/R-induced acute renal injury .TNF-α-induced Fas/FasL pathway may be involved in this process .%目的：探究1，2-二甲磺酸乙烷（ EDS）预处理是否对雄性SD大鼠缺血再灌注肾脏损伤有保护作用及其可能机制。方法：夹闭双侧肾蒂45 min，构建雄性SD大鼠肾脏缺血再灌注模型；48只雄性SD大鼠随机分为6组：空白对照组（blank）、假手术组（sham）、肾脏缺血再灌注组（I／R）、EDS预处理＋肾缺血再灌注组（EDS＋I／R）、EDS预处理＋睾酮＋肾缺血再灌注组（ EDS＋TST＋I／R）和阉割＋肾缺血再灌注组（ Cast＋I／R）。术前1 h及再灌注后24 h采血检测血清黄体生成素、睾酮、血肌酐、血尿素氮以及尿液肾损伤分子1（ KIM-1）水平；取肾脏组织，检测TNF-α水平、Fas mRNA与caspase-3蛋白表达情况。结果：（1） EDS＋I／R与Cast＋I／R组比较，血肌酐和尿素氮无显著差异（P＞0.05），但KIM-1水平却低于Cast＋I／R组（P＜0.05），2组血肌酐、血尿素氮和KIM-1均高于另外3组（P＜0.05）。（2）与术前1 h相比，再灌注术后24 h，EDS＋I／R、Cast＋I／R及EDS＋TST＋I／R组均伴睾酮及黄体生成素下降（P＜0.05）。（3） EDS＋I／R组TNF-α水平、Fas mRNA及caspase-3蛋白表达水平均显著低于Cast＋I／R与I／R组（ P＜0.05）。结论：EDS预处理可大幅降低血清睾酮水平，从而减轻雄性SD大鼠肾脏缺血再灌注损伤，其机制可能与TNF-α介导的Fas／FasL通路有关。
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