目的：研究亚硒酸钠（ Na2 SeO3）对中波紫外线（ UVB）损伤人角质形成细胞的保护作用。方法：培养永生化人角质形成细胞（HaCaT细胞），实验分为4组处理：（1）正常对照组；（2）Na2SeO3组：分别加入1 nmol／L、10 nmol／L、50 nmol／L、100 nmol／L、200 nmol／L和1μmol／L的Na2 SeO3预孵育24 h；（3） UVB组：300、600和900 J／m2 UVB照射；（4）Na2SeO3＋UVB组：Na2SeO3预孵育24 h后进行UVB照射。采用MTT法检测细胞增殖活性，采用流式细胞仪检测300 J／m2 UVB照射后细胞的凋亡率。结果：（1） UVB组与正常对照组比较，细胞增殖活性显著降低（P＜0.05），细胞活性与UVB照射剂量呈负相关；（2）Na2SeO3组与正常对照组比较，细胞增殖活性无明显差异；（3）不同浓度Na2SeO3＋UVB组与UVB组比较，细胞增殖活性增加，差异显著（P＜0.05），其中100 nmol／L组促进细胞增殖作用最强；（4）300 J／m2 UVB照射后，不同浓度Na2 SeO3＋UVB组与UVB组比较，凋亡率下降，差异显著（ P＜0.05），其中100 nmol／L组抑制凋亡作用最强。结论： UVB对角质形成细胞有损伤作用，且与照射剂量呈正相关；Na2 SeO3具有光保护性能，可减轻UVB辐射损伤人角质形成细胞。%AIM: To investigate the protective effect of sodium selenite ( Na2 SeO3 ) on human keratinocytes under ultraviolet-B (UVB) irradiation.METHODS: The cultured HaCaT cells were divided into 4 groups: (1) normal control group;(2) Na2 SeO3 group:pretreated with Na2 SeO3 at doses of 10 nmol/L, 50 nmol/L, 100 nmol/L, 200 nmol/L and 1 μmol/L for 24 h;(3) UVB group: irradiated with UVB at doses of 300, 600 and 900 J/m2; (4) Na2SeO3 +UVB group:after pretreated with Na2SeO3 for 24 h, irradiated with UVB at doses of 300, 600 and 900 J/m2.The cell pro-liferation was detected by MTT assay .The apoptotic rates of HaCaT cells treated with UVB at dose of 300 J/m2 were as-sessed by flow cytometry .RESULTS:Compared with normal control group , the cell proliferation activity in UVB group de-creased significantly ( P<0.05 ) .The cell activity was inversely correlated with the irradiation intensity .No significant difference of the cell activity between Na 2 SeO3 group and normal control group was observed .The cell proliferation in Na2SeO3 +UVB group was higher than that in UVB group significantly (P<0.05).Na2SeO3 at concentration of 100 nmol/L showed the strongest activity to promote cell proliferation .After 300 J/m2 UVB irradiation, the apoptotic rate in Na2SeO3+UVB group decreased significantly ( P<0.05) compared with UVB group .The inhibitory effect of Na 2 SeO3 at concentra-tion of 100 nmol/L on apoptosis was the strongest .CONCLUSION: The damage of human keratinocytes by UVB irradia-tion is in a dose-dependent manner .The photoprotection performance of Na 2 SeO3 reduces the damage of human keratino-cytes induced by UVB irradiation .
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