目的：探讨不同强度微波辐射对大鼠心脏的损伤作用，并初步探讨其作用机制。方法：分别以强度为500、1000、1500和2000 W／m2，频率为2450 MHz微波照射大鼠6 min，照射后6 h取大鼠心脏，检测心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性，透射电镜观察心脏组织超微结构的改变，Muse细胞分析仪检测心肌细胞凋亡，Western blotting检测心肌细胞内cleaved caspase-3蛋白的表达。结果：随着微波辐射强度的增加，大鼠心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性呈现降低趋势，与对照组相比，差异有统计学意义（ P＜0.05）。透射电镜结果显示，微波辐射致细胞线粒体数量减少且形态异常，大部分线粒体溶解空化，基质明显肿胀。心肌细胞凋亡检测结果显示，微波辐射能够诱导大鼠心肌细胞发生凋亡，且随着微波辐射强度的增加，细胞凋亡率呈上升趋势（P＜0.05）。 Western blotting结果显示，微波辐射后心肌细胞内cleaved caspase-3蛋白表达水平随着辐射强度的增加呈上升趋势（ P＜0.05）。结论：微波辐射对大鼠心脏具有明显的损伤作用，能够引起心脏能量代谢出现异常，诱发心肌细胞发生凋亡。%[ ABSTRACT] AIM:To investigate the effect of microwave radiation at different intensities on the rat myocardium and its possible mechanism.METHODS:The rats were radiated by the intensity of 500, 1 000, 1 500 and 2 000 W/m2 with 2 450 MHz microwave for 6 min.The heart tissue was collected 6 h after microwave radiation.ATP and mitochondria complexⅣandⅤwere measured.The changes of the tissue structures were observed under transmission electron micro-scope.The apoptosis of the myocardial cells was detected by a cell analyzer.The protein level of cleaved caspase-3 was de-termined by Western blotting.RESULTS:The concentration of ATP and activity of mitochondria complexⅣandⅤsigni-ficantly decreased compared with control group in the cardiac tissues.The decreased number, morphological abnormalities such as dissolved cavitation, matrix and obvious tumefaction of mitochondria were observed under transmission electron mi-croscope.The microwave radiation induced the apoptosis of myocardial cells in the rats.The cell apoptotic rate and the pro-tein level of cleaved caspase-3 increased with increasing intensity of microwave radiation ( P<0.05 ) .CONCLUSION:Microwave radiation has obvious injury effect on the rat heart, which can cause cardiac energy metabolism dysregulation and cardiac myocyte apoptosis.
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