目的：观察灯盏花素对高糖环境下人视网膜色素上皮细胞株ARPE-19中VEGF、VEGFR2和p-ERK蛋白的表达及对2型糖尿病大鼠视网膜VEGF表达的影响。方法：培养ARPE-19细胞，分为对照组、灯盏花素组、高糖组和高糖＋灯盏花素组，ELISA检测VEGF的分泌水平，Western blot检测细胞VEGF、VEGFR2和p-ERK的蛋白水平；取正常大鼠，随机分为正常对照组、灯盏花素组、糖尿病模型组和糖尿病灯盏花素治疗组，16周后取各组大鼠眼球，观察大鼠视网膜的病理变化并评分，免疫组化观察VEGF的表达情况。结果：（1）细胞实验结果显示，灯盏花素组VEGF、VEGFR2和p-ERK的蛋白水平与正常对照组比较均减低（ P＜0.05）；高糖组细胞上述蛋白水平与正常对照组比较均增加（P＜0.05）；高糖＋灯盏花素组上述蛋白水平与高糖组比较表达均减少（P＜0.05）；（2）大鼠视网膜病理学评分显示糖尿病组与正常对照组、糖尿病灯盏花素治疗组相比差异显著，灯盏花素组与正常对照组相比差异无统计学意义。糖尿病大鼠与正常对照组、灯盏花素组相比，视网膜VEGF的表达增加；糖尿病灯盏花素治疗组中VEGF的表达较糖尿病组有所降低（ P＜0.05）。结论：灯盏花素可以降低ARPE-19细胞VEGF、VEGFR2和p-ERK的蛋白表达水平，抑制糖尿病大鼠视网膜VEGF的表达，提示灯盏花素可能通过降低糖尿病大鼠视网膜细胞和组织中VEGF的表达，缓解其糖尿病视网膜病变进程。%AIM:To evaluate the influence of scutellarin on the expression of vascular endothelial growth factor ( VEGF) in high glucose-treated human retinal pigment epithelial cell line ARPE-19 and to observe the effects of scutellarin on the protein expression of VEGF, p-ERK and VEGFR2 in the retinas of type II diabetic rats.METHODS: Cultured ARPE-19 cells were divided into normal control group, scutellarin group, high glucose group and high glucose+scutellarin group.The protein levels of VEGF, p-ERK and VEGFR2 were measured by Western blot.The VEGF release in ARPE-19 cells was detected by ELISA.Normal rats were randomly divided into normal control group and scutellarin group.Diabetic rat model was established by feeding with high-fat diet and injecting with streptozocin, and randomly divided into diabetes group and diabetes treated with scutellarin group.After 16 weeks, the eyes were removed.The morphological changes of the retinas were observed under light microscope with HE staining, and histopathological score was recorded.The expres-sion of VEGF in the retinas was observed by the method of immunohistochemistry.RESULTS:Compared with normal con-trol group, the protein levels of VEGF, p-ERK and VEGFR2 in the ARPE-19 cells decreased in scutellarin group, but in-creased in high glucose group.The histopathological score of the retinas showed significant difference among diabetes group, diabetes treated with scutellarin group and normal control group, and no significant difference between normal con-trol group and scutellarin group was observed.The expression of VEGF increased in diabetic group and was significantly higher than that in scutellarin treatment group (P<0.05).CONCLUSION:Scutellarin inhibits the increased protein le-vels of VEGF, p-ERK and VEGFR2 in ARPE-19 cells, and decreases the expression of VEGF in the retinas of diabetic rats.The suppression of the diabetic retinopathy development by scutellarin may be partly involved in the ERK/MAPK pathway.
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