AIM:To observe the expression of hypoxia-inducible factor 1 (HIF-1) and neuroglobin (NGB) in piglet cortex during deep hypothermic circulatory arrest.METHODS:Wuzhishan piglets were randomly assigned to car-diopulmonary bypass group ( CPB group) , 40 min of circulatory arrest ( CA) at 18 ℃ without cerebral perfusion ( DHCA group) or with selective antegrade cerebral perfusion ( SACP group) .After 180 min of reperfusion, cortical tissue was har-vested for determining HIF-1αand NGB expression by HE staining, Western blot and real-time PCR.RESULTS:Severer cerebral injury was observed in DHCA group than that in SACP group.After 180 min of reperfusion, HIF-1αprotein and mRNA levels were significantly higher in DHCA group than those in CPB group (P<0.05).Accordingly, SACP animal had higher levels of HIF-1αprotein and mRNA than those in DHCA group (P<0.05).Simultaneously, higher NGB pro-tein and mRNA levels were found in DHCA group than those in CPB group after 180 min of reperfusion ( P<0.05) .The SACP animal had higher levels of NGB protein and mRNA than those in DHCA group (P<0.05).CONCLUSION:Up-regulation of HIF-1 and NGB are involved in the mechanism against cerebral injury resulting from DHCA in the cortex and possibly a part of cerebral protective effect of SACP.%目的：观察深低温停循环（DHCA）下幼猪脑皮质缺氧诱导因子1（HIF-1）与神经珠蛋白（NGB）的表达变化。方法：五指山猪共15头随机分成体外循环组（ CPB组）、深低温停循环组（ DHCA组）与深低温停循环选择性顺行脑灌注组（ SACP组）。建立体外循环后，DHCA组降温至18℃后停循环40 min，SACP组停循环后经无名动脉顺行脑灌注40 min。复灌180 min后取脑皮质组织行HE染色镜检，Western blot及real-time PCR检测HIF-1α、NGB蛋白表达及mRNA水平。结果：HE染色显示与DHCA组相比SACP组脑组织损伤显著减轻。复灌180 min后DHCA组及SACP组脑皮质中HIF-1α的蛋白及mRNA水平均显著高于CPB组（P＜0．05），同时SACP组动物脑组织HIF-1α的蛋白及mRNA水平显著高于DHCA组（P＜0．05）。复灌180 min后DHCA组及SACP组脑皮质中NGB的蛋白及mRNA水平均显著高于CPB组（ P＜0．05），同时SACP组动物脑组织NGB的蛋白及mRNA水平显著高于DHCA组（P＜0．05）。结论：HIF-1与NGB的表达上调参与了脑组织对DHCA脑损伤的反应机制，并可能是SACP脑保护作用的机制之一。
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