目的：探讨清胰汤Ⅱ号冲剂（ QYT）对急性胰腺炎（ acute pancreatitis，AP）小鼠的保护作用及机制。方法：成年C57BL／6小鼠24只，雌雄各半，随机均分为3组。 AP组和AP＋QYT组首先经腹腔注射雨蛙肽（50μg／kg）及脂多糖（10 mg／kg）复制重症AP模型，AP组小鼠给予饮用水灌胃，AP＋QYT组给予QYT灌胃；正常对照组小鼠给予等量生理盐水注射及饮用水灌胃。于末次注药后3h麻醉处死动物。检测和分析胰腺组织的病理改变、肠道细菌总数和分类、Peyer’s结T淋巴细胞亚群的变化、血浆淀粉酶、白细胞介素6（IL-6）、单核细胞趋化蛋白1（ MCP-1）水平以及胰腺和肺脏组织髓过氧化物酶（ MPO）活性等。结果：与对照组相比，AP小鼠的胰腺组织病理学评分、肠道细菌数量、血浆淀粉酶活性、IL-6及MCP-1水平、胰和肺组织的MPO活性都有明显升高（ P＜0.05）；QYT可在一定程度上逆转AP时相关指标的变化（P＜0.05）。小肠Peyer’s结数量在各组无明显差异，但AP组的CD3＋T淋巴细胞百分比较对照组明显降低（P＜0.05），而且，AP组和AP＋QYT组，尤其是后者CD4＋T淋巴细胞百分比和CD4＋／CD8＋比值较对照组明显升高（ P＜0.05）。结论：清胰汤II号冲剂对雨蛙肽和脂多糖诱导的小鼠急性胰腺炎具有明显的拮抗作用，其机制可能与其抑制炎症反应、促进肠内细菌的清除和调节肠道T淋巴细胞功能有关。%[ ABSTRACT ] AIM: To investigate the protect effect of Chinese herbal preparation, Qing Yi TangⅡgranule (QYT), on acute pancreatitis (AP) mice and its mechanism.METHODS:Adult male and female C57BL/6 mice (n=24) were randomly divided into control group, AP group and AP+QYT group.Severe AP was induced by combined intra-peritoneal injection of caerulein (50μg/kg) and lipopolysaccharide (LPS;10 mg/kg).Drinking water or 24%QYT solu-tion was given to the mice in AP group or AP+QYT group by oral gavage.The mice in control group were intraperitoneally injected with equivalent volume of normal saline and gavaged with water.The mice were sacrificed 3 h after the last injec-tion.Severity of AP was assessed by biochemical markers and histology.The plasma level of IL-6 and MCP-1, and lung myeloperoxidase ( MPO) levels were determined for assessing the extent of systemic inflammatory response.The intestinal microflora, T lymphocytes and T-lymphocyte subgroups were examined for assessing the function of the intestinal barrier. RESULTS:Compared with control group, the mice in AP group presented significant increases in pathological histological scores, plasma amylase activity and IL-6 and MCP-1 levels, as well as the MPO activity in the lung and pancreatic tissues. QYT attenuated these changes to some extent.Furthermore, the increased intestinal microflora was significantly reversed by QYT.No difference of the numbers of Peyer’ s patches in small intestine in the 3 groups was observed, but the percentage of CD3 +T lymphocytes decreased significantly in AP group, and increased percentage of CD4 +and CD4 +/CD8 +ratio were found in AP group and AP+QYT group.CONCLUSION: QYT protects against cearulein and LPS-induced acute pancreatitis in mice.The mechanisms may be related to the suppression of the inflammatory response, promoting intestinal bacteria removal, and regulating the functions of T lymphocytes in the intestinal barrier.
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