目的：研究冬凌草甲素对人肺癌NCI-H460细胞侵袭和迁移的影响。方法：将NCI-H460细胞分为高剂量（ HD）组、中剂量（ MD）组、低剂量（ LD）组和对照（ N）组，前3组（实验组）加入不同浓度的冬凌草甲素，N组不加冬凌草甲素，观察细胞生长情况；用MTT法测定细胞增殖；比较细胞侵袭能力和迁移能力；Western blotting检测细胞MMP-2和MMP-9表达。结果：实验组细胞数量低于对照组，细胞增殖受抑制，HD组抑制率为48．94％， MD组为36．17％，LD组为19．15％；实验组穿透滤膜细胞数明显减少，HD组为26．67±5．16，MD组为36．17±5．08，LD组为44．33±5．50；实验组的迁移速率及迁移细胞数均降低；实验组的MMP-2和MMP-9表达水平明显低于N组（P＜0．01）。结论：冬凌草甲素能抑制NCI-H460肺癌细胞的侵袭和迁移，其作用与下调MMP-2和MMP-9表达有关。%[ABSTRACT]AIM:ToinvestigatetheeffectoforidoninontheinvasionandmigrationofhumanlungcancerNCI-H460 cells.METHODS:NCI-H460 cells were divided into high-dose (HD), middle-dose (MD) and low-dose (LD) oridonin groups (cultured with 40, 20 and 10μmol/L of oridonin, respectively, as experimental groups), and normal (N) group ( treated without oridonin as control ) .The cell growth was observed .The cell proliferation was detected by MTT as-say.Boyden chamber was used to determine the cell invasive capacity .The cell migration was also measured .The levels of MMP-2 and MMP-9 were assayed by Western blotting .RESULTS:The cell counts in the experimental groups were lower than that in N group .The cell proliferation was inhibited as the inhibitory rates were 48.94%, 36.17%and 19.15% for HD group, MD group and LD group, respectively.The numbers of the invasive cells were 26.67 ±5.16 for HD group, 36.17 ±5.08 for MD group, and 44.33 ±5.50 for LD group.The migration rates in the experimental groups were lower than that in N group .The expression of MMP-2 and MMP-9 decreased dependent on the oridonin dose as follows: HD group 展开▼
