目的：探讨参松养心胶囊（SSYX）对糖尿病（DM）大鼠心室电生理特性与结构功能变化的影响。方法：雄性SD大鼠45只，随机分为对照组（CTL组，n＝15）、DM组（n＝15）和SSYX组（n＝15）。 DM组和SSYX组采用一次性腹腔注射链脲佐菌素（60 mg/kg）制备糖尿病模型，CTL组则给予腹腔注射生理盐水（1 mL/kg），72 h后检测血糖评价造模结果，将造模成功的动物纳入本研究。 SSYX组连续6周给予SSYX灌胃（1 g· kg-1· d-1）治疗，DM组和CTL组则给予生理盐水灌胃（2 mL· kg-1· d-1）。动物完成药物治疗后，采用超声心动图检测心功能并记录II导联体表心电图；采用放射免疫法检测血浆的内皮素1（ ET-1）水平；随后在整体心脏Langendorff 灌流条件下行离体电生理研究，分别记录和测量左室前游离壁（LAF）心外膜单相动作电位（MAP）和有效不应期（VERP），同时给予Burst快速电刺激用以进行室性心律失常（ VA）的诱发；采用Masson染色对3组动物心肌纤维化程度进行评价。结果：与CTL组相比，DM组心功能下降（ P＜0.01），而QT间期、VERP、动作电位时程、VA诱发率、心肌纤维化程度和血浆ET-1水平均增加（均P＜0.01）；与DM组相比， SSYX组的心功能得到改善（均P＜0.01），且 QT间期、VERP、动作电位时程、VA诱发率、心肌纤维化程度和血浆ET-1水平均降低（均P＜0.05）。结论：SSYX具有减轻DM大鼠心室电重构和结构重构及改善心功能的作用。%[ABSTRACT]AIM:TodeterminetheeffectsofShensongyangxincapsule(SSYX)ontheventricularelectrical properties, structural remodeling and cardiac function in the rats with diabetes mellitus (DM).METHODS:Male SD rats (n=45) were randomly divided into control group (n=15), DM group (n=15) and SSYX group (n=15).The rats in DM group and SSYX group were injected with streptozotocin (60 mg/kg, ip), while the rats in control group were given normal saline (1 mL/kg, ip).The blood samples were collected 72 h after treatment for determining the blood glucose lev-els in DM group and SSYX group .The model rats in SSYX group were administered with SSYX (1 g· kg-1 · d-1 , ig) for 6 weeks, while the other rats received normal saline (2 mL· kg-1· d-1, ig).The echocardiography was used to assess the cardiac function , and the lead II electrocardiogram was also recorded in all the animals .The radioimmunoassay and Masson trichrome staining were used to measure the plasma levels of endothelin -1 (ET-1) and the collagen deposition in the ventricles, respectively.A whole Langendorff-perfused heart model was used to conduct the electrophysiologic study .The monophasic action potential ( MAP) and the ventricular effective refractory period ( VERP) were recorded in the left anteri-or free wall ( LAF) , and the burst pacing was used to induce ventricular arrhythmia ( VA) .RESULTS: Compared with control group, the VERP, action potential duration (APD), QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were increased , while the cardiac function was declined in DM group .Compared with DM group , the VERP, APD, QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were all de-creased, while the cardiac function was improved in SSYX group .CONCLUSION: SSYX attenuates the electrical and structural remodeling and improves the cardiac function in DM rats .
展开▼
