AIM: To investigate the expression of Raf kinase inhibitor protein (RKIP) and phosphorylated extracellular signal-regulated kinase (p-ERK) in the renal tissues of diabetic rats. METHODS: Forty SD rats were randomly divided into normal control group and diabetic nephropathy group. The pathological changes of the renal tissues were observed under microscope with HE and periodic acid-Schiff ( PAS) staining. The expression of RKIP and p-ERKl/2 was detected by the methods of immunohistochemistry and Western blotting. RESULTS: Following the extension of duration, the blood sugar of the rats in diabetic nephropathy group increased, with the appearance of proteinuria, increase in kidney weight, increase in renal p-ERKl/2 expression and decrease in RKIP expression as compared with control group. CONCLUSION: The development of diabetic nephropathy may be accelerated by the decrease in RKIP and the increase in p-ERK.%目的:探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)和磷酸化细胞外信号调节激酶(phosphorylated extracellular signal-regulated kinase,p-ERK)在糖尿病肾病大鼠肾组织中的表达及意义.方法:40只Sprague-Dawley (SD)大鼠随机分为2组:正常对照组(N组)和糖尿病肾病组(M组).苏木精-伊红(hematoxylin-eosin,HE)和过碘酸-雪夫(periodic acid-Schiff,PAS)染色观察肾组织病理改变,免疫组化方法检测各组肾组织RKIP和p-ERK1/2表达,Western blotting 检测各组RKIP和p-ERK1/2的表达.结果:M组大鼠随着病程的延长,血糖升高,出现蛋白尿,肾重增加,肾组织内p-ERK1/2明显增高,RKIP降低,与N组相比有显著差异(P<0.05).结论:RKIP表达的减少及p-ERK的表达增加可能促进糖尿病肾病的进展.
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